TY - JOUR
T1 - Enhanced Activity of Polynuclear Aromatic Hydrocarbon Mixtures as Aryl Hydrocarbon (Ah) Receptor Agonists
AU - Chaloupka, Kathy
AU - Harper, Nyla
AU - Krishnan, Venkatesh
AU - Steinberg, Michael
AU - Santostefano, Michael
AU - Safe, Stephen H.
AU - Womack, James
AU - Rodriguez, Lewis V.
AU - Dunsford, Harold A.
AU - Goldstein, Lawrence S.
PY - 1994/8/1
Y1 - 1994/8/1
N2 - The relative potencies of benzor[a]pyrene and a complex mixture of polycyclic aromatic hydrocarbons (PAH) produced as by-products of manufactured gas plant (MGP) residues as inducers of hepatic microsomal ethoxyresorufin O-deethylase (EROD) were determined in the B6C3F1mouse. The ED50 values for the induction response were 78 and 65 mg/kg for benzo[a]pyrene and the MGP-PAH mixture, respectively, although benzo[a]pyrene and other compounds containing four or more rings were only trace components of this mixture. A comparison of the EROD induction potencies of benzo[a]pyrene and the MGP-PAH mixture showed that the mixture was approximately 706 times more active than expected based on its benzo[a]pyrene content (0.17%). The enhanced activity of the MGP-PAH mixture was also observed for several other aryl hydrocarbon (Ah) receptor-mediated responses, including inhibition of the splenic plaque-forming cell (PFC) response to both T-cell-dependent and independent antigens in B6C3F1mice. The nature of these PAH interactions will be discussed.
AB - The relative potencies of benzor[a]pyrene and a complex mixture of polycyclic aromatic hydrocarbons (PAH) produced as by-products of manufactured gas plant (MGP) residues as inducers of hepatic microsomal ethoxyresorufin O-deethylase (EROD) were determined in the B6C3F1mouse. The ED50 values for the induction response were 78 and 65 mg/kg for benzo[a]pyrene and the MGP-PAH mixture, respectively, although benzo[a]pyrene and other compounds containing four or more rings were only trace components of this mixture. A comparison of the EROD induction potencies of benzo[a]pyrene and the MGP-PAH mixture showed that the mixture was approximately 706 times more active than expected based on its benzo[a]pyrene content (0.17%). The enhanced activity of the MGP-PAH mixture was also observed for several other aryl hydrocarbon (Ah) receptor-mediated responses, including inhibition of the splenic plaque-forming cell (PFC) response to both T-cell-dependent and independent antigens in B6C3F1mice. The nature of these PAH interactions will be discussed.
KW - PAH
KW - hepatic CYP1A1 induction
UR - http://www.scopus.com/inward/record.url?scp=84963333796&partnerID=8YFLogxK
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U2 - 10.1080/10406639408014729
DO - 10.1080/10406639408014729
M3 - Article
AN - SCOPUS:84963333796
VL - 7
SP - 191
EP - 198
JO - Polycyclic Aromatic Compounds
JF - Polycyclic Aromatic Compounds
SN - 1040-6638
IS - 1-3
ER -