Engineered Graphene Oxide Nanocomposite Capable of Preventing the Evolution of Antimicrobial Resistance

Huizhen Zheng; Zhaoxia Ji; Kevin R. Roy; Meng Gao; Yanxia Pan; Xiaoming Cai; Liming Wang; Wei Li; Chong Hyun Chang; Chitrada Kaweeteerawat; Chunying Chen; Tian Xia; Yuliang Zhao; Ruibin Li

doi:10.1021/acsnano.9b04970

Engineered Graphene Oxide Nanocomposite Capable of Preventing the Evolution of Antimicrobial Resistance

Huizhen Zheng, Zhaoxia Ji, Kevin R. Roy, Meng Gao, Yanxia Pan, Xiaoming Cai, Liming Wang, Wei Li, Chong Hyun Chang, Chitrada Kaweeteerawat, Chunying Chen, Tian Xia, Yuliang Zhao, Ruibin Li

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Antimicrobial resistance (AMR) is spreading worldwide and keeps evolving to adapt to antibiotics, causing increasing threats in clinics, which necessitates the exploration of antimicrobial agents for not only killing of resistant cells but also prevention of AMR progression. However, so far, there has been no effective approach. Herein, we designed lanthanum hydroxide and graphene oxide nanocomposites (La@GO) to confer a synergistic bactericidal effect in all tested resistant strains. More importantly, long-term exposure of E. coli (AMR) to subminimum inhibitory concentrations of La@GO does not trigger detectable secondary resistance, while conventional antibiotics and silver nanoparticles lead to a 16-to 64-fold increase in tolerance. The inability of E. coli to evolve resistance to La@GO is likely due to a distinctive extracellular multitarget invasion killing mechanism involving lipid dephosphorylation, lipid peroxidation, and peptidoglycan disruption. Overall, our results highlight La@GO nanocomposites as a promising solution to combating resistant bacteria without inducing the evolution of AMR.

Original language	English (US)
Pages (from-to)	11488-11499
Number of pages	12
Journal	ACS Nano
Volume	13
Issue number	10
DOIs	https://doi.org/10.1021/acsnano.9b04970
State	Published - Oct 22 2019

Keywords

antimicrobial resistance
evolution
graphene oxide
lanthanum hydroxide
nanoparticles

ASJC Scopus subject areas

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)

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10.1021/acsnano.9b04970

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Engineered Graphene Oxide Nanocomposite Capable of Preventing the Evolution of Antimicrobial Resistance. / Zheng, Huizhen; Ji, Zhaoxia; Roy, Kevin R.; Gao, Meng; Pan, Yanxia; Cai, Xiaoming; Wang, Liming; Li, Wei; Chang, Chong Hyun; Kaweeteerawat, Chitrada; Chen, Chunying; Xia, Tian; Zhao, Yuliang; Li, Ruibin.

In: ACS Nano, Vol. 13, No. 10, 22.10.2019, p. 11488-11499.

Research output: Contribution to journal › Article › peer-review

Zheng, Huizhen ; Ji, Zhaoxia ; Roy, Kevin R. ; Gao, Meng ; Pan, Yanxia ; Cai, Xiaoming ; Wang, Liming ; Li, Wei ; Chang, Chong Hyun ; Kaweeteerawat, Chitrada ; Chen, Chunying ; Xia, Tian ; Zhao, Yuliang ; Li, Ruibin. / Engineered Graphene Oxide Nanocomposite Capable of Preventing the Evolution of Antimicrobial Resistance. In: ACS Nano. 2019 ; Vol. 13, No. 10. pp. 11488-11499.

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title = "Engineered Graphene Oxide Nanocomposite Capable of Preventing the Evolution of Antimicrobial Resistance",

abstract = "Antimicrobial resistance (AMR) is spreading worldwide and keeps evolving to adapt to antibiotics, causing increasing threats in clinics, which necessitates the exploration of antimicrobial agents for not only killing of resistant cells but also prevention of AMR progression. However, so far, there has been no effective approach. Herein, we designed lanthanum hydroxide and graphene oxide nanocomposites (La@GO) to confer a synergistic bactericidal effect in all tested resistant strains. More importantly, long-term exposure of E. coli (AMR) to subminimum inhibitory concentrations of La@GO does not trigger detectable secondary resistance, while conventional antibiotics and silver nanoparticles lead to a 16-to 64-fold increase in tolerance. The inability of E. coli to evolve resistance to La@GO is likely due to a distinctive extracellular multitarget invasion killing mechanism involving lipid dephosphorylation, lipid peroxidation, and peptidoglycan disruption. Overall, our results highlight La@GO nanocomposites as a promising solution to combating resistant bacteria without inducing the evolution of AMR.",

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author = "Huizhen Zheng and Zhaoxia Ji and Roy, {Kevin R.} and Meng Gao and Yanxia Pan and Xiaoming Cai and Liming Wang and Wei Li and Chang, {Chong Hyun} and Chitrada Kaweeteerawat and Chunying Chen and Tian Xia and Yuliang Zhao and Ruibin Li",

note = "Funding Information: This work was supported by a grant from the National Natural Science Foundation of China (No. 21976126; No. 21806116) Key Project of Social Development of Jiangsu Province (BE2018653) a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Suzhou Science and Technology Development Project (SYS201715), and the Users with Excellence Project of Hefei Science Center CAS (2018HSC-UE004) Publisher Copyright: Copyright {\textcopyright} 2019 American Chemical Society. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

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AU - Cai, Xiaoming

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AU - Li, Wei

AU - Chang, Chong Hyun

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AU - Zhao, Yuliang

AU - Li, Ruibin

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PY - 2019/10/22

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N2 - Antimicrobial resistance (AMR) is spreading worldwide and keeps evolving to adapt to antibiotics, causing increasing threats in clinics, which necessitates the exploration of antimicrobial agents for not only killing of resistant cells but also prevention of AMR progression. However, so far, there has been no effective approach. Herein, we designed lanthanum hydroxide and graphene oxide nanocomposites (La@GO) to confer a synergistic bactericidal effect in all tested resistant strains. More importantly, long-term exposure of E. coli (AMR) to subminimum inhibitory concentrations of La@GO does not trigger detectable secondary resistance, while conventional antibiotics and silver nanoparticles lead to a 16-to 64-fold increase in tolerance. The inability of E. coli to evolve resistance to La@GO is likely due to a distinctive extracellular multitarget invasion killing mechanism involving lipid dephosphorylation, lipid peroxidation, and peptidoglycan disruption. Overall, our results highlight La@GO nanocomposites as a promising solution to combating resistant bacteria without inducing the evolution of AMR.

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Engineered Graphene Oxide Nanocomposite Capable of Preventing the Evolution of Antimicrobial Resistance

Abstract

Keywords

ASJC Scopus subject areas

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