Endothelial progenitor cell mobilization by preoperative exercise: A bone marrow response associated with postoperative outcome

R. Schier, R. El-Zein, A. Cortes, M. Liu, M. Collins, N. Rafat, P. Teschendorf, Hua Kang Wu, J. Heymach, R. Mehran, B. Riedel

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Background. Preoperative anaemia is associated with increased morbidity in patients undergoing major surgery. Whether erythrocytes are the only bone-marrow-derived cell lineage that associates with increased surgical complications is unknown. This prospective observational trial studied the mobilization of endothelial progenitor cells (EPCs) in response to exercise in association with postoperative complications. Methods. After IRB approval, 60 subjects undergoing major thoracic surgerywere exercised to exhaustion (peak VO2 ). Peripheral blood collected before and after peak exercise was quantified for EPC lineages by fluorescence-activated cell sorter analysis. Complication analysis was based on the Clavien.Dindo classification. Results. Exhaustive exercise increased EPC [CD452133+34+ cells=150 (0.00-5230) to 220 (0.00-1270) cells μl-1; median change (range)=20 (24,180-860) cells μl-1; P=0.03] but not mature endothelial cell (EC) subpopulations. Pre-exercise levels [odds ratio (OR)=0.86, 95% confidence interval (CI): 0.37-2.00, P=0.72), change after exercise as a continuous variable (OR=0.95, 95% CI: 0.41-2.22, P=0.91) and a positive response after exercise (change >0 cells μl-1; OR=0.41, 95% CI: 0.13-1.28, P=0.12) were not statistically significantly associated with the incidence of postoperative complications. Post-hoc receiver operating characteristic curve analyses revealed that subjects with a CD452133+34+ increase ≥60 cells μl-1 in response to exercise suffered fewer postoperative complications [86% sensitivity, 48% specificity and AUC=0.67 (95% CI: 0.52-0.81)]. Conclusions. Preoperative exercise induces EPC into the peripheral circulation. Subjects with a poor EPC response had a pre-existing propensity for postoperative complications. This warrants further research into the role of bone marrow function as a critical component to endothelial repair mechanisms. Clinical trial registration. IRB 2003-0434 (University of Texas M.D. Anderson Cancer Center, Houston, TX, USA).

Original languageEnglish (US)
Pages (from-to)652-660
Number of pages9
JournalBritish journal of anaesthesia
Volume113
Issue number4
DOIs
StatePublished - Oct 1 2014

Keywords

  • Training
  • Vascular

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Fingerprint Dive into the research topics of 'Endothelial progenitor cell mobilization by preoperative exercise: A bone marrow response associated with postoperative outcome'. Together they form a unique fingerprint.

Cite this