TY - JOUR
T1 - Endothelial progenitor cell mobilization by preoperative exercise
T2 - A bone marrow response associated with postoperative outcome
AU - Schier, R.
AU - El-Zein, R.
AU - Cortes, A.
AU - Liu, M.
AU - Collins, M.
AU - Rafat, N.
AU - Teschendorf, P.
AU - Wu, Hua Kang
AU - Heymach, J.
AU - Mehran, R.
AU - Riedel, B.
N1 - Publisher Copyright:
© The Author 2014.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Background. Preoperative anaemia is associated with increased morbidity in patients undergoing major surgery. Whether erythrocytes are the only bone-marrow-derived cell lineage that associates with increased surgical complications is unknown. This prospective observational trial studied the mobilization of endothelial progenitor cells (EPCs) in response to exercise in association with postoperative complications. Methods. After IRB approval, 60 subjects undergoing major thoracic surgerywere exercised to exhaustion (peak VO2 ). Peripheral blood collected before and after peak exercise was quantified for EPC lineages by fluorescence-activated cell sorter analysis. Complication analysis was based on the Clavien.Dindo classification. Results. Exhaustive exercise increased EPC [CD452133+34+ cells=150 (0.00-5230) to 220 (0.00-1270) cells μl-1; median change (range)=20 (24,180-860) cells μl-1; P=0.03] but not mature endothelial cell (EC) subpopulations. Pre-exercise levels [odds ratio (OR)=0.86, 95% confidence interval (CI): 0.37-2.00, P=0.72), change after exercise as a continuous variable (OR=0.95, 95% CI: 0.41-2.22, P=0.91) and a positive response after exercise (change >0 cells μl-1; OR=0.41, 95% CI: 0.13-1.28, P=0.12) were not statistically significantly associated with the incidence of postoperative complications. Post-hoc receiver operating characteristic curve analyses revealed that subjects with a CD452133+34+ increase ≥60 cells μl-1 in response to exercise suffered fewer postoperative complications [86% sensitivity, 48% specificity and AUC=0.67 (95% CI: 0.52-0.81)]. Conclusions. Preoperative exercise induces EPC into the peripheral circulation. Subjects with a poor EPC response had a pre-existing propensity for postoperative complications. This warrants further research into the role of bone marrow function as a critical component to endothelial repair mechanisms. Clinical trial registration. IRB 2003-0434 (University of Texas M.D. Anderson Cancer Center, Houston, TX, USA).
AB - Background. Preoperative anaemia is associated with increased morbidity in patients undergoing major surgery. Whether erythrocytes are the only bone-marrow-derived cell lineage that associates with increased surgical complications is unknown. This prospective observational trial studied the mobilization of endothelial progenitor cells (EPCs) in response to exercise in association with postoperative complications. Methods. After IRB approval, 60 subjects undergoing major thoracic surgerywere exercised to exhaustion (peak VO2 ). Peripheral blood collected before and after peak exercise was quantified for EPC lineages by fluorescence-activated cell sorter analysis. Complication analysis was based on the Clavien.Dindo classification. Results. Exhaustive exercise increased EPC [CD452133+34+ cells=150 (0.00-5230) to 220 (0.00-1270) cells μl-1; median change (range)=20 (24,180-860) cells μl-1; P=0.03] but not mature endothelial cell (EC) subpopulations. Pre-exercise levels [odds ratio (OR)=0.86, 95% confidence interval (CI): 0.37-2.00, P=0.72), change after exercise as a continuous variable (OR=0.95, 95% CI: 0.41-2.22, P=0.91) and a positive response after exercise (change >0 cells μl-1; OR=0.41, 95% CI: 0.13-1.28, P=0.12) were not statistically significantly associated with the incidence of postoperative complications. Post-hoc receiver operating characteristic curve analyses revealed that subjects with a CD452133+34+ increase ≥60 cells μl-1 in response to exercise suffered fewer postoperative complications [86% sensitivity, 48% specificity and AUC=0.67 (95% CI: 0.52-0.81)]. Conclusions. Preoperative exercise induces EPC into the peripheral circulation. Subjects with a poor EPC response had a pre-existing propensity for postoperative complications. This warrants further research into the role of bone marrow function as a critical component to endothelial repair mechanisms. Clinical trial registration. IRB 2003-0434 (University of Texas M.D. Anderson Cancer Center, Houston, TX, USA).
KW - Training
KW - Vascular
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U2 - 10.1093/bja/aeu135
DO - 10.1093/bja/aeu135
M3 - Article
C2 - 24880828
AN - SCOPUS:84908635184
VL - 113
SP - 652
EP - 660
JO - British journal of anaesthesia
JF - British journal of anaesthesia
SN - 0007-0912
IS - 4
ER -