TY - JOUR
T1 - Emergence of a predominant clone of community-acquired Staphylococcus aureus among children in Houston, Texas
AU - Avalos Mishaan, Ana M.
AU - Mason, Edward
AU - Martinez-Aguilar, Gerardo
AU - Hammerman, Wendy
AU - Propst, Jennifer J.
AU - Lupski, James R.
AU - Stankiewicz, Pawel
AU - Kaplan, Sheldon
AU - Hulten, Kristina
PY - 2005/3/1
Y1 - 2005/3/1
N2 - Background: Community-acquired (CA), methicillin-resistant Staphylococcus aureus (MRSA) infections among children are increasing in the United States. At Texas Children's Hospital (TCH), surveillance has been in place since August 2001. The objectives of this study were to describe the distribution of CA S. aureus among patients at TCH and to study genomic relationships of isolates collected between August 2001 and July 2003. Methods: Genomic relationships were determined with repetitive element-polymerase chain reaction (PCR). Multilocus sequence typing was performed for selected strains representing major clones. Molecular characterization of CA-MRSA was performed with PCR, including staphylococcal cassette chromosome (SCCmec), pvl (lukS-PV plus lukF-PV), hla, hlb and selected microbial surface components recognizing adhesive matrix molecule genes, ie, cna, clfA, fnbA and fnbB. Results: A 62% increase was observed in CA S. aureus infections from year 1 (2001-2002) to year 2 (2002-2003), whereas the annual number of hospital admissions was unchanged. CA methicillin-sensitive S. aureus isolates were more likely to be associated with invasive infections than were CA-MRSA isolates (P < 0.01). TCH clone A, sequence type (ST) 8, was responsible for approximately 94% of all CA-MRSA isolated from children in the greater Houston area. Clone A differed from clones B (ST30) and C (ST1) by lacking the cna gene while carrying the fnbB gene. Conclusions: One CA-MRSA clone, TCH clone A, has become the predominant cause of CA S. aureus infections among children in the Houston area. It causes a wide spectrum of diseases, including complicated pneumonia.
AB - Background: Community-acquired (CA), methicillin-resistant Staphylococcus aureus (MRSA) infections among children are increasing in the United States. At Texas Children's Hospital (TCH), surveillance has been in place since August 2001. The objectives of this study were to describe the distribution of CA S. aureus among patients at TCH and to study genomic relationships of isolates collected between August 2001 and July 2003. Methods: Genomic relationships were determined with repetitive element-polymerase chain reaction (PCR). Multilocus sequence typing was performed for selected strains representing major clones. Molecular characterization of CA-MRSA was performed with PCR, including staphylococcal cassette chromosome (SCCmec), pvl (lukS-PV plus lukF-PV), hla, hlb and selected microbial surface components recognizing adhesive matrix molecule genes, ie, cna, clfA, fnbA and fnbB. Results: A 62% increase was observed in CA S. aureus infections from year 1 (2001-2002) to year 2 (2002-2003), whereas the annual number of hospital admissions was unchanged. CA methicillin-sensitive S. aureus isolates were more likely to be associated with invasive infections than were CA-MRSA isolates (P < 0.01). TCH clone A, sequence type (ST) 8, was responsible for approximately 94% of all CA-MRSA isolated from children in the greater Houston area. Clone A differed from clones B (ST30) and C (ST1) by lacking the cna gene while carrying the fnbB gene. Conclusions: One CA-MRSA clone, TCH clone A, has become the predominant cause of CA S. aureus infections among children in the Houston area. It causes a wide spectrum of diseases, including complicated pneumonia.
KW - Clonality
KW - Community-acquired
KW - Methicillin-resistant
KW - Repetitive element-polymerase chain reaction
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=14944387033&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14944387033&partnerID=8YFLogxK
U2 - 10.1097/01.inf.0000151107.29132.70
DO - 10.1097/01.inf.0000151107.29132.70
M3 - Article
C2 - 15750454
AN - SCOPUS:14944387033
SN - 0891-3668
VL - 24
SP - 201
EP - 206
JO - Pediatric Infectious Disease Journal
JF - Pediatric Infectious Disease Journal
IS - 3
ER -