Embryonic stem cell-derived endothelial cells for treatment of hindlimb ischemia

Ngan F. Huang, Hiroshi Niiyama, Abhijit De, Sanjiv S. Gambhir, John P. Cooke

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Peripheral arterial disease (PAD) results from narrowing of the peripheral arteries that supply oxygenated blood and nutrients to the legs and feet, This pathology causes symptoms such as intermittent claudication (pain with walking), painful ischemic ulcerations, or even limb-threatening gangrene. It is generally believed that the vascular endothelium, a monolayer of endothelial cells that invests the luminal surface of all blood and lymphatic vessels, plays a dominant role in vascular homeostasis and vascular regeneration. As a result, stem cell-based regeneration of the endothelium may be a promising approach for treating PAD. In this video, we demonstrate the transplantation of embryonic stem cell (ESC)-derived endothelial cells for treatment of unilateral hindimb ischemia as a model of PAD, followed by non-invasive tracking of cell homing and survival by bioluminescence imaging. The specific materials and procedures for cell delivery and imaging will be described. This protocol follows another publication in describing the induction of hindlimb ischemia by Niiyama et al.1.

Original languageEnglish (US)
Article numbere1034
JournalJournal of Visualized Experiments
Issue number23
StatePublished - Jan 2009


  • Bioluminescence imaging
  • Cell transplantation
  • Embryonic stem cell
  • Hindlimb ischemia
  • Issue 23
  • Medicine
  • Mouse model
  • Non-invasive tracking
  • Peripheral arterial disease

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)


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