Eliminating active species by endohedral metallofullerenol in vitro and in vivo

Fang Lao; Jun Jie Yin; Peter P. Fu; Yuliang Zhao; Xueyun Gao; Paul C. Wang; Chunying Chen; Xing Jie Liang

Eliminating active species by endohedral metallofullerenol in vitro and in vivo

Fang Lao, Jun Jie Yin, Peter P. Fu, Yuliang Zhao, Xueyun Gao, Paul C. Wang, Chunying Chen, Xing Jie Liang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We previously demonstrated that gadolinium endohedral metallofullerenol Gd@C₈₂(OH)₂₂ nanoparticles had high inhibitory activity on growth of malignant tumor in vivo by uncertain mechanism(s). The activities of enzymes associated with the metabolism of reactive oxygen species (ROS) were decreased in the tumor-bearing mice by intraperitoneally injection of Gd@C ₈₂(OH)₂₂ nanoparticles. In current study, systemic investigation of the potential function of Gd@C₈₂(OH)₂₂ nanoparticles found that it expressed direct scavenging activity toward active species. Electron spin resonance (ESR) spectroscopy, the state-of-art technique to measure chemical active species that have one or more unpaired electrons in parallel, was employed to measure free radicals scavenging activities of Gd@C₈₂(OH)₂₂ nanoparticles in vitro. Pre-treatment with Gd@C₈₂(OH)₂₂ nanoparticles significantly reduced ESR signal of various of reactive oxygen species. In addition, Gd@C ₈₂(OH)₂₂ nanoparticles demonstrated extensively scavenging activities of active species measured in vivo, which was consistent with reduced progression of cancer cells treated with Gd@C₈₂(OH) ₂₂ nanoparticles. In summary, results obtained in this study revealed strong active species-scavenging activities of Gd@C₈₂(OH) ₂₂ nanoparticles in vitro and in vivo. Scavenging activities of hydroxylated Gd@C₈₂(OH)₂₂ nanoparticles was systemically measured in different milieu, and demonstrated the inhibition of tumor progression by treatment with Gd@C₈₂(OH)₂₂ nanoparticles likely due to eliminating active species.

Original language	English (US)
Title of host publication	Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
Pages	523-526
Number of pages	4
Volume	2
State	Published - Oct 1 2008
Event	2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 - Quebec City, QC, United States Duration: Jun 1 2008 → Jun 5 2008

Other

Other	2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008
Country	United States
City	Quebec City, QC
Period	6/1/08 → 6/5/08

Keywords

Free radicals
Gd@C(OH)
Scavenging activity

ASJC Scopus subject areas

Mechanical Engineering

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Eliminating active species by endohedral metallofullerenol in vitro and in vivo. / Lao, Fang; Yin, Jun Jie; Fu, Peter P.; Zhao, Yuliang; Gao, Xueyun; Wang, Paul C.; Chen, Chunying; Liang, Xing Jie.

Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. Vol. 2 2008. p. 523-526.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lao, F, Yin, JJ, Fu, PP, Zhao, Y, Gao, X, Wang, PC, Chen, C & Liang, XJ 2008, Eliminating active species by endohedral metallofullerenol in vitro and in vivo. in Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. vol. 2, pp. 523-526, 2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008, Quebec City, QC, United States, 6/1/08.

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title = "Eliminating active species by endohedral metallofullerenol in vitro and in vivo",

abstract = "We previously demonstrated that gadolinium endohedral metallofullerenol Gd@C82(OH)22 nanoparticles had high inhibitory activity on growth of malignant tumor in vivo by uncertain mechanism(s). The activities of enzymes associated with the metabolism of reactive oxygen species (ROS) were decreased in the tumor-bearing mice by intraperitoneally injection of Gd@C 82(OH)22 nanoparticles. In current study, systemic investigation of the potential function of Gd@C82(OH)22 nanoparticles found that it expressed direct scavenging activity toward active species. Electron spin resonance (ESR) spectroscopy, the state-of-art technique to measure chemical active species that have one or more unpaired electrons in parallel, was employed to measure free radicals scavenging activities of Gd@C82(OH)22 nanoparticles in vitro. Pre-treatment with Gd@C82(OH)22 nanoparticles significantly reduced ESR signal of various of reactive oxygen species. In addition, Gd@C 82(OH)22 nanoparticles demonstrated extensively scavenging activities of active species measured in vivo, which was consistent with reduced progression of cancer cells treated with Gd@C82(OH) 22 nanoparticles. In summary, results obtained in this study revealed strong active species-scavenging activities of Gd@C82(OH) 22 nanoparticles in vitro and in vivo. Scavenging activities of hydroxylated Gd@C82(OH)22 nanoparticles was systemically measured in different milieu, and demonstrated the inhibition of tumor progression by treatment with Gd@C82(OH)22 nanoparticles likely due to eliminating active species.",

keywords = "Free radicals, Gd@C(OH), Scavenging activity",

author = "Fang Lao and Yin, {Jun Jie} and Fu, {Peter P.} and Yuliang Zhao and Xueyun Gao and Wang, {Paul C.} and Chunying Chen and Liang, {Xing Jie}",

year = "2008",

month = oct,

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language = "English (US)",

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volume = "2",

pages = "523--526",

booktitle = "Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008",

note = "2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 ; Conference date: 01-06-2008 Through 05-06-2008",

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T1 - Eliminating active species by endohedral metallofullerenol in vitro and in vivo

AU - Lao, Fang

AU - Yin, Jun Jie

AU - Fu, Peter P.

AU - Zhao, Yuliang

AU - Gao, Xueyun

AU - Wang, Paul C.

AU - Chen, Chunying

AU - Liang, Xing Jie

PY - 2008/10/1

Y1 - 2008/10/1

N2 - We previously demonstrated that gadolinium endohedral metallofullerenol Gd@C82(OH)22 nanoparticles had high inhibitory activity on growth of malignant tumor in vivo by uncertain mechanism(s). The activities of enzymes associated with the metabolism of reactive oxygen species (ROS) were decreased in the tumor-bearing mice by intraperitoneally injection of Gd@C 82(OH)22 nanoparticles. In current study, systemic investigation of the potential function of Gd@C82(OH)22 nanoparticles found that it expressed direct scavenging activity toward active species. Electron spin resonance (ESR) spectroscopy, the state-of-art technique to measure chemical active species that have one or more unpaired electrons in parallel, was employed to measure free radicals scavenging activities of Gd@C82(OH)22 nanoparticles in vitro. Pre-treatment with Gd@C82(OH)22 nanoparticles significantly reduced ESR signal of various of reactive oxygen species. In addition, Gd@C 82(OH)22 nanoparticles demonstrated extensively scavenging activities of active species measured in vivo, which was consistent with reduced progression of cancer cells treated with Gd@C82(OH) 22 nanoparticles. In summary, results obtained in this study revealed strong active species-scavenging activities of Gd@C82(OH) 22 nanoparticles in vitro and in vivo. Scavenging activities of hydroxylated Gd@C82(OH)22 nanoparticles was systemically measured in different milieu, and demonstrated the inhibition of tumor progression by treatment with Gd@C82(OH)22 nanoparticles likely due to eliminating active species.

AB - We previously demonstrated that gadolinium endohedral metallofullerenol Gd@C82(OH)22 nanoparticles had high inhibitory activity on growth of malignant tumor in vivo by uncertain mechanism(s). The activities of enzymes associated with the metabolism of reactive oxygen species (ROS) were decreased in the tumor-bearing mice by intraperitoneally injection of Gd@C 82(OH)22 nanoparticles. In current study, systemic investigation of the potential function of Gd@C82(OH)22 nanoparticles found that it expressed direct scavenging activity toward active species. Electron spin resonance (ESR) spectroscopy, the state-of-art technique to measure chemical active species that have one or more unpaired electrons in parallel, was employed to measure free radicals scavenging activities of Gd@C82(OH)22 nanoparticles in vitro. Pre-treatment with Gd@C82(OH)22 nanoparticles significantly reduced ESR signal of various of reactive oxygen species. In addition, Gd@C 82(OH)22 nanoparticles demonstrated extensively scavenging activities of active species measured in vivo, which was consistent with reduced progression of cancer cells treated with Gd@C82(OH) 22 nanoparticles. In summary, results obtained in this study revealed strong active species-scavenging activities of Gd@C82(OH) 22 nanoparticles in vitro and in vivo. Scavenging activities of hydroxylated Gd@C82(OH)22 nanoparticles was systemically measured in different milieu, and demonstrated the inhibition of tumor progression by treatment with Gd@C82(OH)22 nanoparticles likely due to eliminating active species.

KW - Free radicals

KW - Gd@C(OH)

KW - Scavenging activity

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SN - 9781420085075

SN - 9781420085044

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BT - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008

T2 - 2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008

Y2 - 1 June 2008 through 5 June 2008

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