Eliminating active species by endohedral metallofullerenol in vitro and in vivo

Fang Lao, Jun Jie Yin, Peter P. Fu, Yuliang Zhao, Xueyun Gao, Paul C. Wang, Chunying Chen, Xing Jie Liang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We previously demonstrated that gadolinium endohedral metallofullerenol Gd@C82(OH)22 nanoparticles had high inhibitory activity on growth of malignant tumor in vivo by uncertain mechanism(s). The activities of enzymes associated with the metabolism of reactive oxygen species (ROS) were decreased in the tumor-bearing mice by intraperitoneally injection of Gd@C 82(OH)22 nanoparticles. In current study, systemic investigation of the potential function of Gd@C82(OH)22 nanoparticles found that it expressed direct scavenging activity toward active species. Electron spin resonance (ESR) spectroscopy, the state-of-art technique to measure chemical active species that have one or more unpaired electrons in parallel, was employed to measure free radicals scavenging activities of Gd@C82(OH)22 nanoparticles in vitro. Pre-treatment with Gd@C82(OH)22 nanoparticles significantly reduced ESR signal of various of reactive oxygen species. In addition, Gd@C 82(OH)22 nanoparticles demonstrated extensively scavenging activities of active species measured in vivo, which was consistent with reduced progression of cancer cells treated with Gd@C82(OH) 22 nanoparticles. In summary, results obtained in this study revealed strong active species-scavenging activities of Gd@C82(OH) 22 nanoparticles in vitro and in vivo. Scavenging activities of hydroxylated Gd@C82(OH)22 nanoparticles was systemically measured in different milieu, and demonstrated the inhibition of tumor progression by treatment with Gd@C82(OH)22 nanoparticles likely due to eliminating active species.

Original languageEnglish (US)
Title of host publicationTechnical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
Pages523-526
Number of pages4
Volume2
StatePublished - Oct 1 2008
Event2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 - Quebec City, QC, United States
Duration: Jun 1 2008Jun 5 2008

Other

Other2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008
CountryUnited States
CityQuebec City, QC
Period6/1/086/5/08

Keywords

  • Free radicals
  • Gd@C(OH)
  • Scavenging activity

ASJC Scopus subject areas

  • Mechanical Engineering

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