Eliminating active species by endohedral metallofullerenol in vitro and in vivo

Fang Lao; Jun Jie Yin; Peter P. Fu; Yuliang Zhao; Xueyun Gao; Paul C. Wang; Chunying Chen; Xing Jie Liang

Eliminating active species by endohedral metallofullerenol in vitro and in vivo

Fang Lao, Jun Jie Yin, Peter P. Fu, Yuliang Zhao, Xueyun Gao, Paul C. Wang, Chunying Chen, Xing Jie Liang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We previously demonstrated that gadolinium endohedral metallofullerenol Gd@C₈₂(OH)₂₂ nanoparticles had high inhibitory activity on growth of malignant tumor in vivo by uncertain mechanism(s). The activities of enzymes associated with the metabolism of reactive oxygen species (ROS) were decreased in the tumor-bearing mice by intraperitoneally injection of Gd@C ₈₂(OH)₂₂ nanoparticles. In current study, systemic investigation of the potential function of Gd@C₈₂(OH)₂₂ nanoparticles found that it expressed direct scavenging activity toward active species. Electron spin resonance (ESR) spectroscopy, the state-of-art technique to measure chemical active species that have one or more unpaired electrons in parallel, was employed to measure free radicals scavenging activities of Gd@C₈₂(OH)₂₂ nanoparticles in vitro. Pre-treatment with Gd@C₈₂(OH)₂₂ nanoparticles significantly reduced ESR signal of various of reactive oxygen species. In addition, Gd@C ₈₂(OH)₂₂ nanoparticles demonstrated extensively scavenging activities of active species measured in vivo, which was consistent with reduced progression of cancer cells treated with Gd@C₈₂(OH) ₂₂ nanoparticles. In summary, results obtained in this study revealed strong active species-scavenging activities of Gd@C₈₂(OH) ₂₂ nanoparticles in vitro and in vivo. Scavenging activities of hydroxylated Gd@C₈₂(OH)₂₂ nanoparticles was systemically measured in different milieu, and demonstrated the inhibition of tumor progression by treatment with Gd@C₈₂(OH)₂₂ nanoparticles likely due to eliminating active species.

Original language	English (US)
Title of host publication	Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
Pages	523-526
Number of pages	4
State	Published - 2008
Event	2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 - Quebec City, QC, United States Duration: Jun 1 2008 → Jun 5 2008

Publication series

Name	Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
Volume	2

Other

Other	2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008
Country/Territory	United States
City	Quebec City, QC
Period	6/1/08 → 6/5/08

Keywords

Free radicals
Gd@C(OH)
Scavenging activity

ASJC Scopus subject areas

Mechanical Engineering

Cite this

Lao, F., Yin, J. J., Fu, P. P., Zhao, Y., Gao, X., Wang, P. C., Chen, C., & Liang, X. J. (2008). Eliminating active species by endohedral metallofullerenol in vitro and in vivo. In Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008 (pp. 523-526). (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008; Vol. 2).

Eliminating active species by endohedral metallofullerenol in vitro and in vivo. / Lao, Fang; Yin, Jun Jie; Fu, Peter P.; Zhao, Yuliang; Gao, Xueyun; Wang, Paul C.; Chen, Chunying; Liang, Xing Jie.

Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. 2008. p. 523-526 (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lao, F, Yin, JJ, Fu, PP, Zhao, Y, Gao, X, Wang, PC, Chen, C & Liang, XJ 2008, Eliminating active species by endohedral metallofullerenol in vitro and in vivo. in Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008, vol. 2, pp. 523-526, 2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008, Quebec City, QC, United States, 6/1/08.

Lao F, Yin JJ, Fu PP, Zhao Y, Gao X, Wang PC et al. Eliminating active species by endohedral metallofullerenol in vitro and in vivo. In Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. 2008. p. 523-526. (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008).

Lao, Fang ; Yin, Jun Jie ; Fu, Peter P. ; Zhao, Yuliang ; Gao, Xueyun ; Wang, Paul C. ; Chen, Chunying ; Liang, Xing Jie. / Eliminating active species by endohedral metallofullerenol in vitro and in vivo. Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. 2008. pp. 523-526 (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008).

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title = "Eliminating active species by endohedral metallofullerenol in vitro and in vivo",

abstract = "We previously demonstrated that gadolinium endohedral metallofullerenol Gd@C82(OH)22 nanoparticles had high inhibitory activity on growth of malignant tumor in vivo by uncertain mechanism(s). The activities of enzymes associated with the metabolism of reactive oxygen species (ROS) were decreased in the tumor-bearing mice by intraperitoneally injection of Gd@C 82(OH)22 nanoparticles. In current study, systemic investigation of the potential function of Gd@C82(OH)22 nanoparticles found that it expressed direct scavenging activity toward active species. Electron spin resonance (ESR) spectroscopy, the state-of-art technique to measure chemical active species that have one or more unpaired electrons in parallel, was employed to measure free radicals scavenging activities of Gd@C82(OH)22 nanoparticles in vitro. Pre-treatment with Gd@C82(OH)22 nanoparticles significantly reduced ESR signal of various of reactive oxygen species. In addition, Gd@C 82(OH)22 nanoparticles demonstrated extensively scavenging activities of active species measured in vivo, which was consistent with reduced progression of cancer cells treated with Gd@C82(OH) 22 nanoparticles. In summary, results obtained in this study revealed strong active species-scavenging activities of Gd@C82(OH) 22 nanoparticles in vitro and in vivo. Scavenging activities of hydroxylated Gd@C82(OH)22 nanoparticles was systemically measured in different milieu, and demonstrated the inhibition of tumor progression by treatment with Gd@C82(OH)22 nanoparticles likely due to eliminating active species.",

keywords = "Free radicals, Gd@C(OH), Scavenging activity",

author = "Fang Lao and Yin, {Jun Jie} and Fu, {Peter P.} and Yuliang Zhao and Xueyun Gao and Wang, {Paul C.} and Chunying Chen and Liang, {Xing Jie}",

note = "Copyright: Copyright 2008 Elsevier B.V., All rights reserved.; 2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 ; Conference date: 01-06-2008 Through 05-06-2008",

year = "2008",

language = "English (US)",

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AU - Lao, Fang

AU - Yin, Jun Jie

AU - Fu, Peter P.

AU - Zhao, Yuliang

AU - Gao, Xueyun

AU - Wang, Paul C.

AU - Chen, Chunying

AU - Liang, Xing Jie

PY - 2008

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N2 - We previously demonstrated that gadolinium endohedral metallofullerenol Gd@C82(OH)22 nanoparticles had high inhibitory activity on growth of malignant tumor in vivo by uncertain mechanism(s). The activities of enzymes associated with the metabolism of reactive oxygen species (ROS) were decreased in the tumor-bearing mice by intraperitoneally injection of Gd@C 82(OH)22 nanoparticles. In current study, systemic investigation of the potential function of Gd@C82(OH)22 nanoparticles found that it expressed direct scavenging activity toward active species. Electron spin resonance (ESR) spectroscopy, the state-of-art technique to measure chemical active species that have one or more unpaired electrons in parallel, was employed to measure free radicals scavenging activities of Gd@C82(OH)22 nanoparticles in vitro. Pre-treatment with Gd@C82(OH)22 nanoparticles significantly reduced ESR signal of various of reactive oxygen species. In addition, Gd@C 82(OH)22 nanoparticles demonstrated extensively scavenging activities of active species measured in vivo, which was consistent with reduced progression of cancer cells treated with Gd@C82(OH) 22 nanoparticles. In summary, results obtained in this study revealed strong active species-scavenging activities of Gd@C82(OH) 22 nanoparticles in vitro and in vivo. Scavenging activities of hydroxylated Gd@C82(OH)22 nanoparticles was systemically measured in different milieu, and demonstrated the inhibition of tumor progression by treatment with Gd@C82(OH)22 nanoparticles likely due to eliminating active species.

AB - We previously demonstrated that gadolinium endohedral metallofullerenol Gd@C82(OH)22 nanoparticles had high inhibitory activity on growth of malignant tumor in vivo by uncertain mechanism(s). The activities of enzymes associated with the metabolism of reactive oxygen species (ROS) were decreased in the tumor-bearing mice by intraperitoneally injection of Gd@C 82(OH)22 nanoparticles. In current study, systemic investigation of the potential function of Gd@C82(OH)22 nanoparticles found that it expressed direct scavenging activity toward active species. Electron spin resonance (ESR) spectroscopy, the state-of-art technique to measure chemical active species that have one or more unpaired electrons in parallel, was employed to measure free radicals scavenging activities of Gd@C82(OH)22 nanoparticles in vitro. Pre-treatment with Gd@C82(OH)22 nanoparticles significantly reduced ESR signal of various of reactive oxygen species. In addition, Gd@C 82(OH)22 nanoparticles demonstrated extensively scavenging activities of active species measured in vivo, which was consistent with reduced progression of cancer cells treated with Gd@C82(OH) 22 nanoparticles. In summary, results obtained in this study revealed strong active species-scavenging activities of Gd@C82(OH) 22 nanoparticles in vitro and in vivo. Scavenging activities of hydroxylated Gd@C82(OH)22 nanoparticles was systemically measured in different milieu, and demonstrated the inhibition of tumor progression by treatment with Gd@C82(OH)22 nanoparticles likely due to eliminating active species.

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KW - Gd@C(OH)

KW - Scavenging activity

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ER -

Eliminating active species by endohedral metallofullerenol in vitro and in vivo

Abstract

Publication series

Other

Keywords

ASJC Scopus subject areas

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