TY - JOUR
T1 - Electrospun electroconductive constructs of aligned fibers for cardiac tissue engineering
AU - Mancino, Chiara
AU - Hendrickson, Troy
AU - Whitney, Lauren V.
AU - Paradiso, Francesca
AU - Abasi, Sara
AU - Tasciotti, Ennio
AU - Taraballi, Francesca
AU - Guiseppi-Elie, Anthony
N1 - Funding Information:
We thank Gu Jianhua for providing support during SEM images acquisition, Enrica De Rosa and Christopher Tsao for proof-reading the manuscript, and the mentorship., Dr. Jeffrey G. Jacot for providing iPSCs. Support provided by the Center for Bioelectronics, Biosensors, and Biochips (C3B®) and from ABTECH Scientific, Inc. Prof. Guiseppi-Elie acknowledges support via a TEES Research Professorship.
Publisher Copyright:
© 2022
PY - 2022/8
Y1 - 2022/8
N2 - Myocardial infarction remains the leading cause of death in the western world. Since the heart has limited regenerative capabilities, several cardiac tissue engineering (CTE) strategies have been proposed to repair the damaged myocardium. A novel electrospun construct with aligned and electroconductive fibers combining gelatin, poly(lactic-co-glycolic) acid and polypyrrole that may serve as a cardiac patch is presented. Constructs were characterized for fiber alignment, surface wettability, shrinkage and swelling behavior, porosity, degradation rate, mechanical properties, and electrical properties. Cell-biomaterial interactions were studied using three different types of cells, Neonatal Rat Ventricular Myocytes (NRVM), human lung fibroblasts (MRC-5) and induced pluripotent stem cells (iPSCs). All cell types showed good viability and unique organization on construct surfaces depending on their phenotype. Finally, we assessed the maturation status of NRVMs after 14 days by confocal images and qRT-PCR. Overall evidence supports a proof-of-concept that this novel biomaterial construct could be a good candidate patch for CTE applications.
AB - Myocardial infarction remains the leading cause of death in the western world. Since the heart has limited regenerative capabilities, several cardiac tissue engineering (CTE) strategies have been proposed to repair the damaged myocardium. A novel electrospun construct with aligned and electroconductive fibers combining gelatin, poly(lactic-co-glycolic) acid and polypyrrole that may serve as a cardiac patch is presented. Constructs were characterized for fiber alignment, surface wettability, shrinkage and swelling behavior, porosity, degradation rate, mechanical properties, and electrical properties. Cell-biomaterial interactions were studied using three different types of cells, Neonatal Rat Ventricular Myocytes (NRVM), human lung fibroblasts (MRC-5) and induced pluripotent stem cells (iPSCs). All cell types showed good viability and unique organization on construct surfaces depending on their phenotype. Finally, we assessed the maturation status of NRVMs after 14 days by confocal images and qRT-PCR. Overall evidence supports a proof-of-concept that this novel biomaterial construct could be a good candidate patch for CTE applications.
KW - Biomimetic scaffold
KW - Cardiac tissue engineering
KW - Electroconductivity
KW - Electrospinning
KW - Maturation
KW - Myocytes, Cardiac/metabolism
KW - Tissue Engineering/methods
KW - Humans
KW - Biocompatible Materials/metabolism
KW - Cells, Cultured
KW - Pyrroles
KW - Rats
KW - Polymers/metabolism
KW - Tissue Scaffolds
KW - Animals
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U2 - 10.1016/j.nano.2022.102567
DO - 10.1016/j.nano.2022.102567
M3 - Article
C2 - 35595015
AN - SCOPUS:85131042377
SN - 1549-9634
VL - 44
SP - 102567
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
M1 - 102567
ER -