Electronegative LDLs from familial hypercholesterolemic patients are physicochemically heterogeneous but uniformly proapoptotic

Hsin Hung Chen, Brian D. Hosken, Max Huang, John W. Gaubatz, Christine L. Myers, Ronald D. Macfarlane, Henry J. Pownall, Chao Yuh Yang

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

A highly electronegative fraction of human plasma LDLs, designated L5, has distinctive biological activity that includes induction of apoptosis in bovine aortic endothelial cells (BAECs). This study was performed to identify a relationship between LDL density, electronegativity, and biological activity, namely, the induction of apoptosis in BAECs. Plasma LDLs from normolipidemic subjects and homozygotic familial hypercholesterolemia subjects were separated into five subfractions, with increasing electronegativity from L1 to L5, and into seven subfractions according to increasing density, D1 to D7. L1 to L5 were also separated according to density, and D1 to D7 were separated according to charge. The density profiles of L1 to L5 were similar (maximum density = 1.030 ± 0.002 g/ml). Induction of apoptosis by all seven density subfractions was confined to the highly electronegative fraction, L5, and within each density subfraction the magnitude of apoptosis correlated with the L5 content. Electronegative LDL is heterogeneous with respect to density and composition, and induction of apoptosis is more strongly associated with LDL electronegativity than with LDL size or density.

Original languageEnglish (US)
Pages (from-to)177-184
Number of pages8
JournalJournal of lipid research
Volume48
Issue number1
DOIs
StatePublished - Jan 2007

Keywords

  • Apoptosis
  • Atherosclerosis
  • Low density lipoprotein
  • Low density lipoprotein dense subfractions

ASJC Scopus subject areas

  • Endocrinology

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