Electron transfer of hemoglobin at electrodes modified with colloidal clay nanoparticles

Chenghong Lei, Ulla Wollenberger, Nikitas Bistolas, Anthony Guiseppi-Elie, Frieder W. Scheller

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

Nanostructured sodium montmorillonite was prepared via a colloidal chemical approach and deposited onto glassy carbon electrodes (GCE). Subsequently, hemoglobin was spontaneously adsorbed onto the clay membrane-modified electrode. The colloidal clay nanoparticles and the adsorbed protein were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The electrochemical impedance behavior of the system was studied using a microlithographically fabricated interdigitated microsensor electrode (IME). The interaction of the clay nanoparticles with hemoglobin was investigated by UV-VIS spectroscopy and electrochemical methods. The heme protein adsorbed in this way displayed a well-defined electrode process and the electron transfer was confirmed to originate from its heme site. Furthermore, nitric oxide affects the hemoglobin electrochemistry.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalFresenius' Journal of Analytical Chemistry
Volume372
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Clay
  • Direct electrochemistry
  • Hemoglobin
  • Nanomaterials
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry

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