Efficient assessment of developmental, surgical and pathological lymphangiogenesis using a lymphatic reporter mouse and its embryonic stem cells

Mingu Hong, Eunson Jung, Sara Yang, Wonhyuek Jung, Young Jin Seong, Eunkyung Park, Athanasios Bramos, Kyu Eui Kim, Sunju Lee, George Daghlian, Jung In Seo, Inho Choi, In Seon Choi, Chester J. Koh, Agnieszka Kobielak, Qi Long Ying, Maxwell Johnson, Daniel Gardner, Alex K. Wong, Dongwon ChoiYoung Kwon Hong

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Several lymphatic reporter mouse lines have recently been developed to significantly improve imaging of lymphatic vessels. Nonetheless, the usage of direct visualization of lymphatic vessels has not been fully explored and documented. Here, we characterized a new Prox1-tdTomato transgenic lymphatic reporter mouse line, and demonstrated how this animal tool enables the researchers to efficiently assess developmental, surgical and pathological lymphangiogenesis by direct visualization of lymphatic vessels. Moreover, we have derived embryonic stem cells from this reporter line, and successfully differentiated them into lymphatic vessels in vivo. In conclusion, these experimental tools and techniques will help advance lymphatic research.

Original languageEnglish (US)
Article numbere0157126
JournalPLoS ONE
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2016

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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