Efficacy of p16 and ProExC immunostaining in the detection of high-grade cervical intraepithelial neoplasia and cervical carcinoma

Ming Guo, Amy C. Baruch, Elvio G. Silva, Yee Jee Jan, E. Lin, Nour Sneige, Michael T. Deavers

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

We compared the efficacy of p16 and ProExC immunostaining in detecting cervical intraepithelial neoplasia (CIN) 2+ in 136 formalin-fixed, paraffinembedded cervical tissue specimens with consensus diagnoses of normal cervix, CIN 1, CIN 2, CIN 3, and carcinoma. Diffuse staining patterns of more than half the thickness of CINs and more than 10% of carcinoma cells were scored as positive. The positivity of p16 and ProExC increased significantly with the severity of cervical lesion (P <.001). For CIN 2+ or CIN 3+, p16 immunostaining was more sensitive (79% for CIN 2+; 90% for CIN 3+) than ProExC immunostaining (67% for CIN 2+; 84% for CIN 3+). ProExC showed higher specificity for CIN 3+ compared with p16. Specimens with p16+/ProExC+ results showed the highest specificity (100% for CIN 2+; 93% for CIN 3+), suggesting that these 2 biomarkers can be used together to distinguish CIN 2/3 from its mimics in cervical biopsy specimens.

Original languageEnglish (US)
Pages (from-to)212-220
Number of pages9
JournalAmerican Journal of Clinical Pathology
Volume135
Issue number2
DOIs
StatePublished - Feb 2011

Keywords

  • CIN
  • Cervical carcinoma
  • Cervical intraepithelial neoplasia
  • HPV
  • Human papillomavirus
  • P16
  • ProExC

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Efficacy of p16 and ProExC immunostaining in the detection of high-grade cervical intraepithelial neoplasia and cervical carcinoma'. Together they form a unique fingerprint.

Cite this