TY - JOUR
T1 - Effects of some common inducers on the hepatic microsomal metabolism of androstenedione in rainbow trout with special reference to cytochrome P-450-dependent enzymes
AU - Hansson, Tiiu
AU - Rafter, Joseph
AU - Gustafsson, Jan Åke
N1 - Funding Information:
Acknowledgements-Thisw ork was supportedb y grants from the Swedish Medical ResearchC ouncil (No. 13X-2819)a nd from the National Swedish EnvironmentP ro-tection Board. J. Rafter is grateful to Stiftelsen Bengt LundqvistsM inne for a fellowship.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1980/2/15
Y1 - 1980/2/15
N2 - The effects of 16α-cyanopregnenolone, 3-methylcholanthrene (3-MC), Clophen A 50 (Cl 50) and phenobarbital on the total amount of cytochrome P-450 and the metabolism of 4-androstene-3,17-dione in liver microsomes from rainbow trout were studied. Only 3-MC and CI 50 caused marked changes in the cytochrome P-450 levels and the cytochrome P-450-dependent steroid hydroxylase activities. A varying response to 3-MC and Cl 50 was seen in fish of different ages and sex. Different responses of 6β-hydroxylase towards α-naphtoflavone, SKF 525-A and metyrapone in vitro were seen in 3-MC- and Cl 50-treated fish when compared to control fish. It is suggested that endocrine factors may be involved in the regulation of cytochrome P-450-mediated metabolism in fish and the presence of multiple forms of cytochrome P-450 in trout liver is indicated.
AB - The effects of 16α-cyanopregnenolone, 3-methylcholanthrene (3-MC), Clophen A 50 (Cl 50) and phenobarbital on the total amount of cytochrome P-450 and the metabolism of 4-androstene-3,17-dione in liver microsomes from rainbow trout were studied. Only 3-MC and CI 50 caused marked changes in the cytochrome P-450 levels and the cytochrome P-450-dependent steroid hydroxylase activities. A varying response to 3-MC and Cl 50 was seen in fish of different ages and sex. Different responses of 6β-hydroxylase towards α-naphtoflavone, SKF 525-A and metyrapone in vitro were seen in 3-MC- and Cl 50-treated fish when compared to control fish. It is suggested that endocrine factors may be involved in the regulation of cytochrome P-450-mediated metabolism in fish and the presence of multiple forms of cytochrome P-450 in trout liver is indicated.
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U2 - 10.1016/0006-2952(80)90380-9
DO - 10.1016/0006-2952(80)90380-9
M3 - Article
C2 - 6768364
AN - SCOPUS:0018921795
SN - 0006-2952
VL - 29
SP - 583
EP - 587
JO - Biochemical pharmacology
JF - Biochemical pharmacology
IS - 4
ER -