TY - JOUR
T1 - Effects of 2,5-hexanedione on NGF expression in motor and sensory neuronal cells of rats
AU - Liu, Qiang
AU - Liu, Qing Jun
AU - Duan, Hua Wei
AU - Zhang, Yan Shu
AU - Le, Weidong
AU - He, Fengsheng
AU - Zheng, Yuxin
N1 - Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PY - 2005/5
Y1 - 2005/5
N2 - OBJECTIVE: To investigate the effects of 2,5-Hexanedione (2,5-HD), a metabolite of n-hexane,on nerve growth factor (NGF) expression in motor and sensory neuronal cells of peripheral nervous system of rats. METHODS: Primary cultured dorsal root ganglion (DRG) neurons were obtained from new born rats on day 4. The cell line VSC4.1 were cultured. The growth condition of cells were observed with a phase contrast microscope. Immunohistochemistry technology was used to analyze the NGF expression in both VSC4.1 cells and primary cultured DRG cells treated with 4 different dosages of 2, 5-HD (2.5, 5.0, 10.0, 20.0 mmol/L) respectively for 24 hours. RESULTS: The NGF expression decreased significantly in both DRG neurons and VSC4.1 cells dosed respectively with 5.0,10.0,20.0 mmol/L 2,5-HD in comparison with the control group (P < 0.05), with a tendency of dose-effect correlation. CONCLUSION: The results suggested that 2,5-HD can decrease the endogenous NGF levels in both cultured DRG sensory neurons and VSC4.1 motor neuron cells of rats.
AB - OBJECTIVE: To investigate the effects of 2,5-Hexanedione (2,5-HD), a metabolite of n-hexane,on nerve growth factor (NGF) expression in motor and sensory neuronal cells of peripheral nervous system of rats. METHODS: Primary cultured dorsal root ganglion (DRG) neurons were obtained from new born rats on day 4. The cell line VSC4.1 were cultured. The growth condition of cells were observed with a phase contrast microscope. Immunohistochemistry technology was used to analyze the NGF expression in both VSC4.1 cells and primary cultured DRG cells treated with 4 different dosages of 2, 5-HD (2.5, 5.0, 10.0, 20.0 mmol/L) respectively for 24 hours. RESULTS: The NGF expression decreased significantly in both DRG neurons and VSC4.1 cells dosed respectively with 5.0,10.0,20.0 mmol/L 2,5-HD in comparison with the control group (P < 0.05), with a tendency of dose-effect correlation. CONCLUSION: The results suggested that 2,5-HD can decrease the endogenous NGF levels in both cultured DRG sensory neurons and VSC4.1 motor neuron cells of rats.
UR - http://www.scopus.com/inward/record.url?scp=34548454742&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548454742&partnerID=8YFLogxK
M3 - Article
C2 - 16111026
AN - SCOPUS:34548454742
SN - 1000-8020
VL - 34
SP - 271
EP - 274
JO - Wei sheng yan jiu = Journal of hygiene research
JF - Wei sheng yan jiu = Journal of hygiene research
IS - 3
ER -