Effects of 2,5-hexanedione on NGF expression in motor and sensory neuronal cells of rats

Qiang Liu, Qing Jun Liu, Hua Wei Duan, Yan Shu Zhang, Weidong Le, Fengsheng He, Yuxin Zheng

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

OBJECTIVE: To investigate the effects of 2,5-Hexanedione (2,5-HD), a metabolite of n-hexane,on nerve growth factor (NGF) expression in motor and sensory neuronal cells of peripheral nervous system of rats. METHODS: Primary cultured dorsal root ganglion (DRG) neurons were obtained from new born rats on day 4. The cell line VSC4.1 were cultured. The growth condition of cells were observed with a phase contrast microscope. Immunohistochemistry technology was used to analyze the NGF expression in both VSC4.1 cells and primary cultured DRG cells treated with 4 different dosages of 2, 5-HD (2.5, 5.0, 10.0, 20.0 mmol/L) respectively for 24 hours. RESULTS: The NGF expression decreased significantly in both DRG neurons and VSC4.1 cells dosed respectively with 5.0,10.0,20.0 mmol/L 2,5-HD in comparison with the control group (P < 0.05), with a tendency of dose-effect correlation. CONCLUSION: The results suggested that 2,5-HD can decrease the endogenous NGF levels in both cultured DRG sensory neurons and VSC4.1 motor neuron cells of rats.

Original languageEnglish (US)
Pages (from-to)271-274
Number of pages4
JournalWei sheng yan jiu = Journal of hygiene research
Volume34
Issue number3
StatePublished - Jan 1 2005
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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