Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as an antiestrogen in MCF-7 human breast cancer cells

V. Krishnan; T. R. Narisimhan; S. Safe

doi:10.1016/0045-6535(92)90089-A

Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as an antiestrogen in MCF-7 human breast cancer cells

V. Krishnan, T. R. Narisimhan, S. Safe

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The effects of 1 nM 17β-estradiol, 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and their combination on the formation of intracellular cytosolic 52- and 34-kDa proteins was determined using a commercially available cathepsin D radioimmunoassay procedure (ELSA cath-D™ kit) which recognizes both the 34- and 52-kDa proteins. In control (ethanol-treated) cells, the 52-kDa protein levels were 305 ± 49 fmol/mg protein (100%) whereas in cells treated with 1 nM 17β-estradiol, 1 nM TCDD or their combination the levels were 1223 ± 142 (401%), 478 ± 54 (150%) and 227 ± 33 (74%). These results confirm that TCDD inhibits the 17β-estradiol-induced intracellular formation of the 52-/34-kDa proteins and further extends the antiestrogenic properties of this compound.

Original language	English (US)
Pages (from-to)	947-950
Number of pages	4
Journal	Chemosphere
Volume	25
Issue number	7-10
DOIs	https://doi.org/10.1016/0045-6535(92)90089-A
State	Published - 1992

ASJC Scopus subject areas

Environmental Engineering
Environmental Chemistry
General Chemistry
Pollution
Health, Toxicology and Mutagenesis

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10.1016/0045-6535(92)90089-A

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@article{4721e4d6e05446119ae0b6787472788e,

title = "Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as an antiestrogen in MCF-7 human breast cancer cells",

abstract = "The effects of 1 nM 17β-estradiol, 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and their combination on the formation of intracellular cytosolic 52- and 34-kDa proteins was determined using a commercially available cathepsin D radioimmunoassay procedure (ELSA cath-D{\texttrademark} kit) which recognizes both the 34- and 52-kDa proteins. In control (ethanol-treated) cells, the 52-kDa protein levels were 305 ± 49 fmol/mg protein (100\%) whereas in cells treated with 1 nM 17β-estradiol, 1 nM TCDD or their combination the levels were 1223 ± 142 (401\%), 478 ± 54 (150\%) and 227 ± 33 (74\%). These results confirm that TCDD inhibits the 17β-estradiol-induced intracellular formation of the 52-/34-kDa proteins and further extends the antiestrogenic properties of this compound.",

author = "V. Krishnan and Narisimhan, \{T. R.\} and S. Safe",

note = "Funding Information: ACKNOWLEDGEMENTS The financial assistance of the National Institutes of Health (P42-ES04917 and ES04176) and the Texas Agricultural Experiment Station are gratefully acknowledged. S. Safe is a Burroughs Wellcome Toxicology Scholar. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1992",

doi = "10.1016/0045-6535(92)90089-A",

language = "English (US)",

volume = "25",

pages = "947--950",

journal = "Chemosphere",

issn = "0045-6535",

publisher = "Elsevier Ltd",

number = "7-10",

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TY - JOUR

T1 - Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as an antiestrogen in MCF-7 human breast cancer cells

AU - Krishnan, V.

AU - Narisimhan, T. R.

AU - Safe, S.

N1 - Funding Information: ACKNOWLEDGEMENTS The financial assistance of the National Institutes of Health (P42-ES04917 and ES04176) and the Texas Agricultural Experiment Station are gratefully acknowledged. S. Safe is a Burroughs Wellcome Toxicology Scholar. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1992

Y1 - 1992

N2 - The effects of 1 nM 17β-estradiol, 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and their combination on the formation of intracellular cytosolic 52- and 34-kDa proteins was determined using a commercially available cathepsin D radioimmunoassay procedure (ELSA cath-D™ kit) which recognizes both the 34- and 52-kDa proteins. In control (ethanol-treated) cells, the 52-kDa protein levels were 305 ± 49 fmol/mg protein (100%) whereas in cells treated with 1 nM 17β-estradiol, 1 nM TCDD or their combination the levels were 1223 ± 142 (401%), 478 ± 54 (150%) and 227 ± 33 (74%). These results confirm that TCDD inhibits the 17β-estradiol-induced intracellular formation of the 52-/34-kDa proteins and further extends the antiestrogenic properties of this compound.

AB - The effects of 1 nM 17β-estradiol, 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and their combination on the formation of intracellular cytosolic 52- and 34-kDa proteins was determined using a commercially available cathepsin D radioimmunoassay procedure (ELSA cath-D™ kit) which recognizes both the 34- and 52-kDa proteins. In control (ethanol-treated) cells, the 52-kDa protein levels were 305 ± 49 fmol/mg protein (100%) whereas in cells treated with 1 nM 17β-estradiol, 1 nM TCDD or their combination the levels were 1223 ± 142 (401%), 478 ± 54 (150%) and 227 ± 33 (74%). These results confirm that TCDD inhibits the 17β-estradiol-induced intracellular formation of the 52-/34-kDa proteins and further extends the antiestrogenic properties of this compound.

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U2 - 10.1016/0045-6535(92)90089-A

DO - 10.1016/0045-6535(92)90089-A

M3 - Article

AN - SCOPUS:0027048622

SN - 0045-6535

VL - 25

SP - 947

EP - 950

JO - Chemosphere

JF - Chemosphere

IS - 7-10

ER -

Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as an antiestrogen in MCF-7 human breast cancer cells

Abstract

ASJC Scopus subject areas

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