TY - JOUR
T1 - Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as an antiestrogen in MCF-7 human breast cancer cells
AU - Krishnan, V.
AU - Narisimhan, T. R.
AU - Safe, S.
N1 - Funding Information:
ACKNOWLEDGEMENTS The financial assistance of the National Institutes of Health (P42-ES04917 and ES04176) and the Texas Agricultural Experiment Station are gratefully acknowledged. S. Safe is a Burroughs Wellcome Toxicology Scholar.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992
Y1 - 1992
N2 - The effects of 1 nM 17β-estradiol, 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and their combination on the formation of intracellular cytosolic 52- and 34-kDa proteins was determined using a commercially available cathepsin D radioimmunoassay procedure (ELSA cath-D™ kit) which recognizes both the 34- and 52-kDa proteins. In control (ethanol-treated) cells, the 52-kDa protein levels were 305 ± 49 fmol/mg protein (100%) whereas in cells treated with 1 nM 17β-estradiol, 1 nM TCDD or their combination the levels were 1223 ± 142 (401%), 478 ± 54 (150%) and 227 ± 33 (74%). These results confirm that TCDD inhibits the 17β-estradiol-induced intracellular formation of the 52-/34-kDa proteins and further extends the antiestrogenic properties of this compound.
AB - The effects of 1 nM 17β-estradiol, 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and their combination on the formation of intracellular cytosolic 52- and 34-kDa proteins was determined using a commercially available cathepsin D radioimmunoassay procedure (ELSA cath-D™ kit) which recognizes both the 34- and 52-kDa proteins. In control (ethanol-treated) cells, the 52-kDa protein levels were 305 ± 49 fmol/mg protein (100%) whereas in cells treated with 1 nM 17β-estradiol, 1 nM TCDD or their combination the levels were 1223 ± 142 (401%), 478 ± 54 (150%) and 227 ± 33 (74%). These results confirm that TCDD inhibits the 17β-estradiol-induced intracellular formation of the 52-/34-kDa proteins and further extends the antiestrogenic properties of this compound.
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U2 - 10.1016/0045-6535(92)90089-A
DO - 10.1016/0045-6535(92)90089-A
M3 - Article
AN - SCOPUS:0027048622
SN - 0045-6535
VL - 25
SP - 947
EP - 950
JO - Chemosphere
JF - Chemosphere
IS - 7-10
ER -