Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as an antiestrogen in MCF-7 human breast cancer cells

V. Krishnan, T. R. Narisimhan, S. Safe

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The effects of 1 nM 17β-estradiol, 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and their combination on the formation of intracellular cytosolic 52- and 34-kDa proteins was determined using a commercially available cathepsin D radioimmunoassay procedure (ELSA cath-D™ kit) which recognizes both the 34- and 52-kDa proteins. In control (ethanol-treated) cells, the 52-kDa protein levels were 305 ± 49 fmol/mg protein (100%) whereas in cells treated with 1 nM 17β-estradiol, 1 nM TCDD or their combination the levels were 1223 ± 142 (401%), 478 ± 54 (150%) and 227 ± 33 (74%). These results confirm that TCDD inhibits the 17β-estradiol-induced intracellular formation of the 52-/34-kDa proteins and further extends the antiestrogenic properties of this compound.

Original languageEnglish (US)
Pages (from-to)947-950
Number of pages4
JournalChemosphere
Volume25
Issue number7-10
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

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