TY - JOUR
T1 - Effect of vitamin A on the growth and differentiation of rat external auditory canal epithelium in organ culture
AU - Liauw, Lishya
AU - LIm, David J.
AU - Bakaletz, Lauren O.
AU - van Blitterswijk, Clemens
N1 - Funding Information:
yngology, Otological Research Laboratories, Ohio State University, Columbus, OH; and the Department of Otolaryngology, University of Leiden, Leiden, The Netherlands. Accepted for publication January 23, 1991. Presented in part at the Thirteenth Midwinter Research Meeting of the Association for Research in Otolaryngology, St Petersburg Beach, FL, Febuary 4-8, 1990. Supported by NINCDS/NIHgrant NS08854, the Mgr. C. van Overbeek stichting, ZEISS and STIR. Address correspondence and reprint requests to David J. Lim, MD, Otological Research Laboratories 4531 University Hospitals Clinic, 456 10th Ave, Columbus. OH 43210. Copyright 0 1991 by W.B. Saunders Company 0196-0709/91/1202-0006$5.00/O
PY - 1991
Y1 - 1991
N2 - Intact rat external ear canal explants were maintained either in retinoid-deficient or retinoid-supplemented medium for 7, 10, 14, and 21 days. The morphology of the external ear canal epidermis was well maintained, including the presence of sebaceous glands even after 21 days in culture with retinoid-deficient medium. However, after culturing in retinoid-supplemented medium, the external ear canal epithelium showed both a loss of keratohyalin granules and the formation of keratin. Extensive microvilli formation occurred, even though the desquamation process continued from the superficial layer after 7, 10, and 14 days in culture with retinoid-enriched medium. After 21 days in culture with retinoid-supplemented medium, the ear canal epithelium contained well-developed Golgi apparatus and secretory granules as well. It was concluded that the ear canal epithelium was transformed into a secretory-like mucosal epithelium by retinoid supplementation.
AB - Intact rat external ear canal explants were maintained either in retinoid-deficient or retinoid-supplemented medium for 7, 10, 14, and 21 days. The morphology of the external ear canal epidermis was well maintained, including the presence of sebaceous glands even after 21 days in culture with retinoid-deficient medium. However, after culturing in retinoid-supplemented medium, the external ear canal epithelium showed both a loss of keratohyalin granules and the formation of keratin. Extensive microvilli formation occurred, even though the desquamation process continued from the superficial layer after 7, 10, and 14 days in culture with retinoid-enriched medium. After 21 days in culture with retinoid-supplemented medium, the ear canal epithelium contained well-developed Golgi apparatus and secretory granules as well. It was concluded that the ear canal epithelium was transformed into a secretory-like mucosal epithelium by retinoid supplementation.
KW - external auditory canal
KW - light microscopy
KW - organ culture
KW - rat
KW - transmission microscopy
KW - vitamin A
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U2 - 10.1016/0196-0709(91)90039-I
DO - 10.1016/0196-0709(91)90039-I
M3 - Article
C2 - 1713425
AN - SCOPUS:0026006619
SN - 0196-0709
VL - 12
SP - 67
EP - 75
JO - American Journal of Otolaryngology--Head and Neck Medicine and Surgery
JF - American Journal of Otolaryngology--Head and Neck Medicine and Surgery
IS - 2
ER -