Effect of temperature on permeation of low-density lipoprotein particles through human carotid artery tissues

Mohamad G. Ghosn, Michael Leba, Astha Vijayananda, Panteha Rezaee, Joel D. Morrisett, Kirill V. Larin

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Quantification of the diffusion of small molecules and large lipid transporting lipoproteins across arterial tissues could be useful in elucidating the mechanism(s) of athero-sclerosis. Optical coherence tomography (OCT) was used to determine the effect of temperature on the rate of diffusion of glucose and low-density lipoproteins (LDL) in human carotid endarterectomy tissue in vitro. The permeability rate for glucose was calculated to be (3.51 ± 0.27) ×10-5cm/s (n = 13) at 20 ° C, and (3.70 ± 0.44)×10-5 cm/s (n = 5) at 37 °C; for LDL the rate was (2.42 ± 0.33)×10-5cm/s (n = 5) at 20 ° C and (4.77 ± 0.48)×10-5cm/s (n = 7) at 37 ° C, where n is the number of samples. These results demonstrate that tem-perature does not significantly influence the permeation of small molecules (e.g. glucose), however, raising the temperature does significantly increase the permeation of LDL. These results provide new information about the capacity of an atherogenic lipoprotein to traverse the intimal layer of the artery. These results also demonstrate the potential of OCT for elucidating the dynamics of lipoprotein perfusion across the arterial wall.

Original languageEnglish (US)
Pages (from-to)573-580
Number of pages8
JournalJournal of Biophotonics
Issue number10
StatePublished - Oct 2009


  • Atherosclerosis
  • Low-density lipoproteins
  • Optical coherence tomography
  • Permeability rate

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)


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