Effect of prostaglandin e2 and bacterial endotoxin on the rate of dye transport in the eustachian tube of the chinchilla

Lauren O. Bakaletz, Stephen R. Griffith, David J. Lim

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

To determine the effects of various biologic agents on the rate of fluid transport in the eustachian tube of the chinchilla, we have established an in situ method of measuring dye transport in which the bulla remains intact. The normal rate of dye transport from an injection site in the superior bulla to the nasopharyngeal orifice of the eustachian tube was 130 ± 10 seconds. Inhibition of ciliary activity with the local anesthetic bupivacaine resulted in a saturable delay of transport (> 15 minutes), while exposure to the β-adrenergic stimulator isoproterenol caused a significant increase in transport rate (79 ± 7 seconds). Two inflammatory mediators commonly found in chronic middle ear effusions were also tested for their effect on fluid transport by the mucociliary apparatus. Bacterial endotoxin reduced transport rates at high concentrations, while prostaglandin E2 had no effect at any concentration tested.

Original languageEnglish (US)
Pages (from-to)278-282
Number of pages5
JournalAnnals of Otology, Rhinology & Laryngology
Volume98
Issue number4
DOIs
StatePublished - Apr 1989

Keywords

  • endotoxin
  • eustachian tube
  • middle ear
  • mucociliary transport
  • prostaglandin E

ASJC Scopus subject areas

  • Otorhinolaryngology

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