Effect of mercuric and silver ions on cesium sulfate equilibrium buoyant densities of synthetic polydeoxyribonucleotides

Michael W. Lieberman, Donald J. Harvan, David E. Amacher, James B. Patterson

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The effect of Hg2+ and Ag+ on the buoyant density (ρ{variant}) of four synthetic DNA polymers, poly[d(A-T)]; poly(dA) · poly(dT); poly[d(G-C)]; and poly(dG) · poly(dC), was investigated. The buoyant density of poly[d(A-T)] in Cs2SO4 increased dramatically after complexing with Hg2+, but little change in the buoyant density of other polymers resulted except at very high molar ratios of Hg2+ DNA-P (rf) Hg2+ raised the thermal transition temperature (Tm) of alternating polymers and lowered the Tm of homopolymers. Measurements in the preparative ultracentrifuge indicated that lowered Tm correlated with Hg2+-induced strand separation of one homopolymer [poly(dA) · poly(dT)], but strand separation was not observed with another homopolymer [poly(dG) · poly(dC)] complexed with Hg2+. When Ag+ was mixed with the polymers, the buoyant density of poly(dG) · poly(dC) increased most markedly. A substantial increase in the buoyant density of poly[d(A-T)] and a small increase in the buoyant density of poly[d(G-C)] were also observed. The Tm changes induced by Ag+ were not related in any obvious way to buoyant density changes. These findings indicate that nucleotide sequence as well as overall base composition is of importance in understanding the buoyant density changes induced by metal ions. Although these data do not allow construction of a detailed molecular model of polymer-metal ion interactions, they may be used to explain much of the behavior of naturally occurring DNA sequences, such as heterochromatic satellite sequences and 5 S and rRNA sequences, in Hg2+/Cs2SO4 and Ag+/Cs2SO4 gradients.

Original languageEnglish (US)
Pages (from-to)265-277
Number of pages13
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume425
Issue number3
DOIs
StatePublished - Mar 17 1976

ASJC Scopus subject areas

  • Medicine(all)

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