Effect of lysophosphatidylcholine on vasomotor functions of porcine coronary arteries

Rakesh Safaya, Hong Chai, Panagiotis Kougias, Peter Lin, Alan B. Lumsden, Qizhi Yao, Changyi Chen

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Background. Lysophosphatidylcholine (LPC) is a product of phosphatidylcholine hydrolysis by phospholipase A2 and a mediator of the lipid-induced atherosclerotic changes. In this study, we determined the effects of LPC on vasomotor functions, oxidative stress, and endothelial nitric oxide synthase (eNOS) expression in porcine coronary arteries. Methods. Porcine coronary arteries were cut into 5-mm rings and were treated with LPC or antioxidant selenomethionine (SeMet). For the vasomotor studies, we used a myograph tension system. Levels of superoxide anion (O2 -) were detected by the lucigenin-enhanced chemiluminescence method. The eNOS protein level was studied by immunohistochemistry with avidin-biotin complex immunoperoxidase procedure. Results. Endothelium-dependent relaxation in response to bradykinin was reduced by 36% and 81% for the rings treated with 12.5 and 25 μm of LPC, respectively, as compared with controls (P < 0.05). Endothelium-independent relaxation in response to sodium nitroprusside also was reduced by 63% after treatment with 25 μm LPC (P < 0.05). The O 2 - level was increased in the porcine arteries treated with 25 μm of LPC by 41% as compared with controls (P < 0.05). The antioxidant SeMet reversed the effects of LPC on vascular relaxation and O 2 - production. Immunoreactivity of eNOS in LPC-treated vessel rings also was reduced substantially. Conclusions. LPC impairs endothelium-dependent and endothelium-independent vasorelaxation. This effect is associated with increased superoxide radical production and decreased eNOS activity and is practically reversed with the use of the antioxidant SeMet.

Original languageEnglish (US)
Pages (from-to)182-188
Number of pages7
JournalJournal of Surgical Research
Volume126
Issue number2
DOIs
StatePublished - Jun 15 2005

Keywords

  • Coronary arteries
  • Endothelial dysfunction
  • Endothelial nitric oxide synthase
  • Lysophosphatidylcholine
  • Oxidative stress
  • Porcine
  • Superoxide anion

ASJC Scopus subject areas

  • Surgery

Fingerprint Dive into the research topics of 'Effect of lysophosphatidylcholine on vasomotor functions of porcine coronary arteries'. Together they form a unique fingerprint.

Cite this