TY - JOUR
T1 - Effect of low-level laser irradiation on osteoblast-like cells cultured on porous hydroxyapatite scaffolds
AU - Parenti, Serena Incerti
AU - Panseri, Silvia
AU - Gracco, Antonio
AU - Sandri, Monica
AU - Tampieri, Anna
AU - Bonetti, Giulio Alessandri
PY - 2013/12/23
Y1 - 2013/12/23
N2 - Objective. To determine the effect of laser irradiation at a low dose on human osteoblast-like cells.Materials and methods. 32 porous hydroxyapatite scaffolds currently used for bone tissue engineering were seeded with MG63 cells and irradiated or not with a GaAlAs diode laser (wavelength 915 nm, dose 2 J/cm2) using different power density and exposure duration.Results. After 72-h incubation, cells showed well spread morphology and good adhesion on both laser-treated and untreated scaffolds. Laser irradiation did not interfere in cell viability and proliferation as compared with the non-irradiated controls.Conclusion. This study suggests that there is no effect of 915 nm laser irradiation at a dose of 2 J/cm2 on the proliferation rate of MG63 cells. Future investigations are needed to compare different dose and wavelength regimens in order to determine the optimal set of laser parameters for maximum cell yield and safe clinical application.
AB - Objective. To determine the effect of laser irradiation at a low dose on human osteoblast-like cells.Materials and methods. 32 porous hydroxyapatite scaffolds currently used for bone tissue engineering were seeded with MG63 cells and irradiated or not with a GaAlAs diode laser (wavelength 915 nm, dose 2 J/cm2) using different power density and exposure duration.Results. After 72-h incubation, cells showed well spread morphology and good adhesion on both laser-treated and untreated scaffolds. Laser irradiation did not interfere in cell viability and proliferation as compared with the non-irradiated controls.Conclusion. This study suggests that there is no effect of 915 nm laser irradiation at a dose of 2 J/cm2 on the proliferation rate of MG63 cells. Future investigations are needed to compare different dose and wavelength regimens in order to determine the optimal set of laser parameters for maximum cell yield and safe clinical application.
KW - Biomaterials
KW - Human osteoblast-like cells
KW - Low-level laser therapy
KW - Porous hydroxyapatite scaffolds
KW - Three-dimensional cultures
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U2 - 10.4415/ANN_13_03_04
DO - 10.4415/ANN_13_03_04
M3 - Article
C2 - 24071604
AN - SCOPUS:84890503922
SN - 0021-2571
VL - 49
SP - 255
EP - 260
JO - Annali dell'Istituto Superiore di Sanita
JF - Annali dell'Istituto Superiore di Sanita
IS - 3
ER -