Effect of low-level laser irradiation on osteoblast-like cells cultured on porous hydroxyapatite scaffolds

Serena Incerti Parenti, Silvia Panseri, Antonio Gracco, Monica Sandri, Anna Tampieri, Giulio Alessandri Bonetti

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Objective. To determine the effect of laser irradiation at a low dose on human osteoblast-like cells.Materials and methods. 32 porous hydroxyapatite scaffolds currently used for bone tissue engineering were seeded with MG63 cells and irradiated or not with a GaAlAs diode laser (wavelength 915 nm, dose 2 J/cm2) using different power density and exposure duration.Results. After 72-h incubation, cells showed well spread morphology and good adhesion on both laser-treated and untreated scaffolds. Laser irradiation did not interfere in cell viability and proliferation as compared with the non-irradiated controls.Conclusion. This study suggests that there is no effect of 915 nm laser irradiation at a dose of 2 J/cm2 on the proliferation rate of MG63 cells. Future investigations are needed to compare different dose and wavelength regimens in order to determine the optimal set of laser parameters for maximum cell yield and safe clinical application.

Original languageEnglish (US)
Pages (from-to)255-260
Number of pages6
JournalAnnali dell'Istituto Superiore di Sanita
Volume49
Issue number3
DOIs
StatePublished - Dec 23 2013

Keywords

  • Biomaterials
  • Human osteoblast-like cells
  • Low-level laser therapy
  • Porous hydroxyapatite scaffolds
  • Three-dimensional cultures

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

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