TY - JOUR
T1 - Effect of hydroxyapatite porosity on growth and differentiation of human osteoblast-like cells
AU - Specchia, N.
AU - Pagnotta, A.
AU - Cappella, M.
AU - Tampieri, A.
AU - Greco, F.
N1 - Funding Information:
We are grateful to Ms Sandra Manzotti for her histological and immunohistological technical assistance. This work was supported by CNR (Consiglio Nazionale delle Ricerche) funds (Project MTSA 2).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002/2/1
Y1 - 2002/2/1
N2 - To study whether hydroxyapatite (HA) porosity can influence its osteoconductive properties, cell adhesion, proliferation and differentiation were compared in human osteoblast-like cells grown on HA disks of different porosity (A = 20%, B = 40%, C = 60%). Human osteoblast-like cells were isolated and characterized. Proliferation rate and alkaline phosphatase (ALP) activity were assessed at 3, 7, 15, 21, and 28 days. Type I collagen and osteonectin production were demonstrated with fluorescence microscopy and osteoblast adhesion studied at 7 and 21 days by scanning electron microscopic analysis. Cell growth on HA was three- to six-fold lower than on polystyrene control disks. At 28 days, 2141 (±350) cells/well grew on the most porous disks (Group C), with highly significant differences from controls (p <0.005). The ALP production was 2-3 fold lower on HA than on plastic. In the Group C the mean ALP activity was of 2.95 (±0.07) UI/well after 28 days, higher than in the other two groups. At 21 and 28 days, proliferation rate and ALP activity on the three HA cultures were significantly different (p < 0.05). A decrease in cell population and increased ALP activity were observed on the most porous material, and high proliferation and poor differentiation rates on the less porous disks.
AB - To study whether hydroxyapatite (HA) porosity can influence its osteoconductive properties, cell adhesion, proliferation and differentiation were compared in human osteoblast-like cells grown on HA disks of different porosity (A = 20%, B = 40%, C = 60%). Human osteoblast-like cells were isolated and characterized. Proliferation rate and alkaline phosphatase (ALP) activity were assessed at 3, 7, 15, 21, and 28 days. Type I collagen and osteonectin production were demonstrated with fluorescence microscopy and osteoblast adhesion studied at 7 and 21 days by scanning electron microscopic analysis. Cell growth on HA was three- to six-fold lower than on polystyrene control disks. At 28 days, 2141 (±350) cells/well grew on the most porous disks (Group C), with highly significant differences from controls (p <0.005). The ALP production was 2-3 fold lower on HA than on plastic. In the Group C the mean ALP activity was of 2.95 (±0.07) UI/well after 28 days, higher than in the other two groups. At 21 and 28 days, proliferation rate and ALP activity on the three HA cultures were significantly different (p < 0.05). A decrease in cell population and increased ALP activity were observed on the most porous material, and high proliferation and poor differentiation rates on the less porous disks.
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U2 - 10.1023/A:1013725809480
DO - 10.1023/A:1013725809480
M3 - Article
AN - SCOPUS:0036471787
SN - 0022-2461
VL - 37
SP - 577
EP - 584
JO - Journal of Materials Science
JF - Journal of Materials Science
IS - 3
ER -