Abstract
The effect of high hydrostatic pressure on the mechanism of transfer for lipophiles between membranes was investigated. The transfer of fluorescent phosphatidylcholine (1-myristoyl-2- left bracket 9 prime -(3 prime -pyrenyl)nonanoyl right bracket phosphatidylcholine) between recombinant complexes of apolipoprotein A-I and 1-palimitoyl-2-oleoylphosphatidylcholine occurred via the aqueous compartment. High hydrostatic pressure retarded the rate of transfer, resulting in a positive activation volume for the reaction. The calculated free energy of activation was approximately 23 kcal/mol. The positive activation volume presumably corresponds to the work necessary to overcome pressure-induced compression of the membrane (protein-lipid complex) before transfer can occur.
Original language | English (US) |
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Pages (from-to) | 3317-3319 |
Number of pages | 3 |
Journal | Journal of the American Chemical Society |
Volume | 106 |
Issue number | 11 |
DOIs | |
State | Published - Oct 1984 |
ASJC Scopus subject areas
- Catalysis
- General Chemistry
- Biochemistry
- Colloid and Surface Chemistry