TY - JOUR
T1 - Effect of hormones on growth and ATP content of a human prostatic carcinoma cell line, LNCaP‐r
AU - Hasenson, Michael
AU - Pousette, ÅKe
AU - Hartley‐åsp, Beryl
AU - Kihlfors, Christer
AU - Lundin, Arne
AU - Gustafsson, Jan‐ÅKe
PY - 1985
Y1 - 1985
N2 - The use of ATP content as a measurement for cell growth was evaluated in the LNCaP prostatic cancer cell line. ATP content was found to correlate well with cell counts and was an easy and reliable method for following the effect of substances on cell growth. During cultivation for 9 days no effect on cell counts or ATP content could be seen when testosterone (10-10 to 10-6 M), estradiol-17β (10-10 to 10-5 M), 5α-DHT (10-9 to 10 -6 M), prolactin, vitamin A, or antiandrogen was added to the cell medium in different combinations. However, a weak positive effect was seen on the mitotic index when 10 or 100 nM 5α-DHT was added to the cells, whereas 1 μM 5α-DHT inhibited cell growth. Thus despite the fact that this LNCaP line contained 16 fmol androgen receptor/mg protein (K(d) 0.6 nM), it is unresponsive to hormones and should be designated LNCaP-r (resistant). Chromosome analysis revealed that a shift in the modal chromosome number had occurred from the original LNCaP line, which could account for the lack of hormonal sensitivity.
AB - The use of ATP content as a measurement for cell growth was evaluated in the LNCaP prostatic cancer cell line. ATP content was found to correlate well with cell counts and was an easy and reliable method for following the effect of substances on cell growth. During cultivation for 9 days no effect on cell counts or ATP content could be seen when testosterone (10-10 to 10-6 M), estradiol-17β (10-10 to 10-5 M), 5α-DHT (10-9 to 10 -6 M), prolactin, vitamin A, or antiandrogen was added to the cell medium in different combinations. However, a weak positive effect was seen on the mitotic index when 10 or 100 nM 5α-DHT was added to the cells, whereas 1 μM 5α-DHT inhibited cell growth. Thus despite the fact that this LNCaP line contained 16 fmol androgen receptor/mg protein (K(d) 0.6 nM), it is unresponsive to hormones and should be designated LNCaP-r (resistant). Chromosome analysis revealed that a shift in the modal chromosome number had occurred from the original LNCaP line, which could account for the lack of hormonal sensitivity.
KW - ATP
KW - androgen receptor
KW - cancer treatment
KW - eukaryotic cell line
KW - prostatic carcinoma
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U2 - 10.1002/pros.2990070208
DO - 10.1002/pros.2990070208
M3 - Article
C2 - 4048015
AN - SCOPUS:0022355741
VL - 7
SP - 183
EP - 194
JO - Prostate
JF - Prostate
SN - 0270-4137
IS - 2
ER -