Effect of high intensity focused ultrasound on platelet activation, aggregation and adhesion

Sandra L. Poliachik, Pierre D. Mourad, Lawrence A. Crum, Wayne Chandler

Research output: Contribution to journalConference articlepeer-review


High intensity focused ultrasound (HIFU) is being considered as a non-invasive method to halt internal bleeding, thus we investigated the capability of HIFU to produce `acoustic primary hemostasis,' including platelet activation, aggregation and adhesion to a collagen-coated surface. Various HIFU doses were applied to platelet rich plasma (PRP) with and without ultrasound contrast agents (UCA). Flow cytometry, laser aggregometry, conventional microscopy, environmental scanning electron microscopy (ESEM) and passive cavitation detection were used to quantify platelet activation, aggregation, adhesion and associated cavitation. Our results have shown that HIFU can cause platelets in a PRP sample to activate, aggregate and adhere to a collagen-coated surface. Furthermore, UCA (0.1% by volume) can enhance the platelet activity and increase the amount of cavitation induced by HIFU. HIFU exposure alone can cause platelet activity, but the addition of UCA increases the amount and rate of cavitation and platelet activity; therefore cavitation is a likely mechanism of HIFU induced platelet activity.

Original languageEnglish (US)
JournalUltrasound in Medicine and Biology
Issue numberSUPPL. 2
StatePublished - Dec 3 2000
EventThe 9th Congress of World Federation for Ultrasound in Medicine and Biology - Florence, Italy
Duration: May 6 2000May 10 2000

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics


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