Effect of blood collection and processing on radioimmunoassay results for apolipoprotein A-I in plasma

S. A. Brown, C. E. Rhodes, K. Dunn, Antonio Gotto, W. Patsch

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

We studied the effects of different procedures of blood collection and processing on quantification of apolipoprotein A-I (apoA-I) by radioimmunoassay. ApoA-II and apolipoproteins of low- and very-low-density lipoproteins did not cross react in the assay. Analytical recovery of apoA-I at different doses was complete. ApoA-I concentration in pooled human plasma was stable for as long as a year stored at -70°C. Inter- and intra-assay CVs averaged 7% and 5%, respectively. We collected blood from 20 subjects into tubes containing EDTA alone or EDTA with antiproteolytic agents, then separated the plasma either immediately or after 3 h at 4°C. We tested various formulations of antibacterial, antiproteolytic, and anti-oxidant agents added to plasma, measuring apoA-I concentrations either within 24 h of blood collection or after storage of plasma for 6 weeks at -70°C. No significant difference in the concentrations of apoA-I was found in these specimens, regardless of the conditions studied. We condlude that addition of protective agents other than EDTA is not necessary during blood collection or specimen processing for reliable quantification of apoA-I in fresh or frozen human plasma.

Original languageEnglish (US)
Pages (from-to)920-924
Number of pages5
JournalClinical Chemistry
Volume34
Issue number5
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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