Effect of age of cell suspension cultures on susceptibility to a fungal elicitor

Izydor Apostol, Philip Low, Peter Heinstein

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Fungal elicitor induced phytoalexin formation and the corresponding fluorescence transitions of the molecular probes pyranine and oxonol VI, in soybean (Glycine max Merr var Kent) and cotton (Gossypium arboreum L. Nanking) cell suspensions were both significantly affected by the age of the cells. During the lag phase and the beginning of the exponential growth phase both cultures exhibited stress responses (i.e. phytoalexin formation and molecular probe fluorescence transitions) in the absence of added elicitors. This behavior was termed autoelicitation because elicitation occurred without added external stimuli. In contrast, cells in the late exponential-early stationary phase were relatively unresponsive to elicitor. During intermediate growth periods the cell suspensions behaved optimally, producing no phytoalexins until stimulated with an elicitor. It would appear, therefore, that the culture period can be divided into 3 phases, with respect to susceptibility to fungal elicitors: a distinct autoelicitation period (immediately after transfer of the cells into fresh medium), followed by a period in which negligible amounts of phytoalexins are synthesized without elicitor, and culminating in a late period in which the cells respond poorly to elicitor. The onset and duration of these periods are somewhat different for soybean and cotton cells.

Original languageEnglish (US)
Pages (from-to)692-695
Number of pages4
JournalPlant Cell Reports
Volume7
Issue number8
DOIs
StatePublished - Mar 1 1989

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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