Dysregulated cyclin D1 expression early in head and neck tumorigenesis in vivo evidence for an association with subsequent gene amplification

Julie G. Izzo, Vali A. Papadimitrakopoulou, Xue Q. Li, Heladio Ibarguen, Jin S. Lee, Jae Y. Ro, Adel El-Naggar, Waun K. Hong, Walter N. Hittelman

Research output: Contribution to journalArticlepeer-review

117 Scopus citations


Cyclin D1 proto-oncogene is a key regulator of the mammalian cell-cycle acting at the restriction point in late G1. Amplification of the cyclin D1 locus, located on chromosome 11q13, as well as cyclin D1 protein overexpression have been reported in several human malignancies. The purpose of this study was to evaluate cyclin D1 gene copy status and protein expression during the multistep process of head and neck tumorigenesis, using a combination of fluorescence in situ hybridization and immunohistochemistry techniques. From 29 selected patients presenting with head and neck squamous carcinoma and whose tumor cytospins had been previously screened for presence (16 cases) or absence (13 cases) of amplification at the 11q13 band, we analysed 46 paraffin-embedded tissue specimens that demonstrated, besides the primary tumor, the presence of contiguous adjacent normal tissue and/or premalignant lesions. Of the 16 amplified cases, nine demonstrated a continuous progression from premalignant to invasive carcinoma and seven (77.7%) of these cases showed cyclin D1 gene amplification in premalignant lesions prior to development of invasive carcinoma. Increased cyclin D1 protein expression was observed in all 16 amplified tumors and five of the 13 (38.4%) non-amplified tumors. Interestingly, dysregulated cyclin D1 expression was also found in the premalignant lesions adjacent to all 16 amplified tumors, and it appeared to precede cyclin D1 gene amplification. In contrast no dysregulated expression was detected in the premalignant lesions of the non-amplified tumors. In conclusion, these findings provide strong evidence for early dysregulation of cyclin D1 expression during the tumorigenesis process and suggest that dysregulated increased expression precedes and possibly enables gene amplification.

Original languageEnglish (US)
Pages (from-to)2313-2322
Number of pages10
Issue number18
StatePublished - Nov 5 1998


  • Amplification
  • Cyclin D1
  • Dysregulated expression
  • FISH
  • Immunohistochemistry
  • Tumorigenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics


Dive into the research topics of 'Dysregulated cyclin D1 expression early in head and neck tumorigenesis in vivo evidence for an association with subsequent gene amplification'. Together they form a unique fingerprint.

Cite this