Dynamics of dense electronegative low density lipoproteins and their preferential association with lipoprotein phospholipase A2

John W. Gaubatz, Baiba K. Gillard, John B. Massey, Ron C. Hoogeveen, Max Huang, Eric E. Lloyd, Joe L. Raya, Chao Yuh Yang, Henry J. Pownall

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Small, dense, electronegative low density lipoprotein [LDL(-)] is increased in patients with familial hypercholesterolemia and diabetes, populations at increased risk for coronary artery disease. It is present to a lesser extent in normolipidemic subjects. The mechanistic link between small, dense LDL(-) and atherogenesis is not known. To begin to address this, we studied the composition and dynamics of small, dense LDL(-) from normolipidemic subjects. NEFA levels, which correlate with triglyceride content, are quantitatively linked to LDL electronegativity. Oxidized LDL is not specific to small, dense LDL(-) or lipoprotein [a] (i.e., abnormal lipoprotein). Apolipoprotein C-III is excluded from the most abundant LDL (i.e., that of intermediate density: 1.034 < d < 1.050 g/ml) but associated with both small and large LDL(-). In contrast, lipoprotein-associated phospholipase A2 (LpPLA2) is highly enriched only in small, dense LDL(-). The association of LpPLA2 with LDL may occur through amphipathic helical domains that are displaced from the LDL surface by contraction of the neutral lipid core.

Original languageEnglish (US)
Pages (from-to)348-357
Number of pages10
JournalJournal of lipid research
Volume48
Issue number2
DOIs
StatePublished - Feb 2007

Keywords

  • Apo B-100
  • Atherogenesis
  • Fatty acid

ASJC Scopus subject areas

  • Endocrinology

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