TY - JOUR
T1 - Dynamical changing patterns of glycogen and enzyme histochemical activities in rat liver graft undergoing warm ischemia injury
AU - He, Xiao Shun
AU - Ma, Yi
AU - Wu, Lin Wei
AU - Wu, Jin Lang
AU - Hu, Rui De
AU - Chen, Gui Hua
AU - Huang, Jie Fu
N1 - Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2005/5/7
Y1 - 2005/5/7
N2 - Aim: To investigate the changing patterns of glycogen and enzyme histochemical activities in rat liver graft under a different warm schemia time (WIT) and to predict the tolerant time limitation of the liver graft to warm ischemia injury. Methods: The rats were randomized into five groups, WIT was 0, 15, 30, 45, 60 min, respectively, and histochemical staining of liver graft specimens was observed. The recovery changes of glycogen and enzyme histochemistry activities were measured respectively 6 and 24 h following liver graft implantation. Results: The activities of succinic dehydrogenase, cytochrome oxidase, apyrase (Mg++-ATPase) and content of glycogen were decreased gradually after different WIT in a time-dependent manner. The changes were significant when WIT was over 30 min. Conclusion: Hepatic injury is reversible within 30 min of warm ischemia injury. Glycogen and enzyme histochemistry activities of liver grafts and their recovery potency after reperfusion may serve as criteria to evaluate the quality of liver grafts.
AB - Aim: To investigate the changing patterns of glycogen and enzyme histochemical activities in rat liver graft under a different warm schemia time (WIT) and to predict the tolerant time limitation of the liver graft to warm ischemia injury. Methods: The rats were randomized into five groups, WIT was 0, 15, 30, 45, 60 min, respectively, and histochemical staining of liver graft specimens was observed. The recovery changes of glycogen and enzyme histochemistry activities were measured respectively 6 and 24 h following liver graft implantation. Results: The activities of succinic dehydrogenase, cytochrome oxidase, apyrase (Mg++-ATPase) and content of glycogen were decreased gradually after different WIT in a time-dependent manner. The changes were significant when WIT was over 30 min. Conclusion: Hepatic injury is reversible within 30 min of warm ischemia injury. Glycogen and enzyme histochemistry activities of liver grafts and their recovery potency after reperfusion may serve as criteria to evaluate the quality of liver grafts.
KW - Histochemical stain
KW - Liver transplantation
KW - Warm ischemia injury
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U2 - 10.3748/wjg.v11.i17.2662
DO - 10.3748/wjg.v11.i17.2662
M3 - Article
C2 - 15849830
AN - SCOPUS:18944389117
VL - 11
SP - 2662
EP - 2665
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
SN - 1007-9327
IS - 17
ER -