Dynamical changing patterns of glycogen and enzyme histochemical activities in rat liver graft undergoing warm ischemia injury

Xiao Shun He, Yi Ma, Lin Wei Wu, Jin Lang Wu, Rui De Hu, Gui Hua Chen, Jie Fu Huang

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Aim: To investigate the changing patterns of glycogen and enzyme histochemical activities in rat liver graft under a different warm schemia time (WIT) and to predict the tolerant time limitation of the liver graft to warm ischemia injury. Methods: The rats were randomized into five groups, WIT was 0, 15, 30, 45, 60 min, respectively, and histochemical staining of liver graft specimens was observed. The recovery changes of glycogen and enzyme histochemistry activities were measured respectively 6 and 24 h following liver graft implantation. Results: The activities of succinic dehydrogenase, cytochrome oxidase, apyrase (Mg++-ATPase) and content of glycogen were decreased gradually after different WIT in a time-dependent manner. The changes were significant when WIT was over 30 min. Conclusion: Hepatic injury is reversible within 30 min of warm ischemia injury. Glycogen and enzyme histochemistry activities of liver grafts and their recovery potency after reperfusion may serve as criteria to evaluate the quality of liver grafts.

Original languageEnglish (US)
Pages (from-to)2662-2665
Number of pages4
JournalWorld Journal of Gastroenterology
Volume11
Issue number17
DOIs
StatePublished - May 7 2005

Keywords

  • Histochemical stain
  • Liver transplantation
  • Warm ischemia injury

ASJC Scopus subject areas

  • Gastroenterology

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