Dynamic tether force measurements of the outer hair cells plasma membrane with optical tweezers

B. Anvari; Z. Li; Masayoshi Takashima; P. Brecht; J. H. Torres; W. E. Brownell

doi:10.1117/12.426765

Dynamic tether force measurements of the outer hair cells plasma membrane with optical tweezers

B. Anvari, Z. Li, Masayoshi Takashima, P. Brecht, J. H. Torres, W. E. Brownell

Research output: Contribution to journal › Article

2 Scopus citations

Abstract

The mechanical characteristics of the outer hair cells (OHCs) plasma membrane was studied by forming tethers with optical tweezers. The average force to from a plasma membrane tether at the lateral wall of the OHC was about 3.5 times greater than that at the basal end of the cell, consistent with the presence of a more extensive cytoskeleton support beneath the PM at the site of the lateral wall. The apparent viscosity of the PM was measured by pulling tethers at different rates while continuously recording the tether force, and was estimated in the range of 13-33 pN•s/μm.

Original language	English (US)
Pages (from-to)	103-108
Number of pages	6
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4260
DOIs	https://doi.org/10.1117/12.426765
State	Published - Jan 1 2001

Keywords

Biomechanics
Cell
Electromotility
Hearing
Lasers
Viscosity

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.426765

Fingerprint Dive into the research topics of 'Dynamic tether force measurements of the outer hair cells plasma membrane with optical tweezers'. Together they form a unique fingerprint.

Cite this

@article{9c0852066ce34264ad80d4d30a5dbe96,

title = "Dynamic tether force measurements of the outer hair cells plasma membrane with optical tweezers",

abstract = "The mechanical characteristics of the outer hair cells (OHCs) plasma membrane was studied by forming tethers with optical tweezers. The average force to from a plasma membrane tether at the lateral wall of the OHC was about 3.5 times greater than that at the basal end of the cell, consistent with the presence of a more extensive cytoskeleton support beneath the PM at the site of the lateral wall. The apparent viscosity of the PM was measured by pulling tethers at different rates while continuously recording the tether force, and was estimated in the range of 13-33 pN•s/μm.",

keywords = "Biomechanics, Cell, Electromotility, Hearing, Lasers, Viscosity",

author = "B. Anvari and Z. Li and Masayoshi Takashima and P. Brecht and Torres, {J. H.} and Brownell, {W. E.}",

year = "2001",

month = jan,

day = "1",

doi = "10.1117/12.426765",

language = "English (US)",

volume = "4260",

pages = "103--108",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

}

TY - JOUR

T1 - Dynamic tether force measurements of the outer hair cells plasma membrane with optical tweezers

AU - Anvari, B.

AU - Li, Z.

AU - Takashima, Masayoshi

AU - Brecht, P.

AU - Torres, J. H.

AU - Brownell, W. E.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - The mechanical characteristics of the outer hair cells (OHCs) plasma membrane was studied by forming tethers with optical tweezers. The average force to from a plasma membrane tether at the lateral wall of the OHC was about 3.5 times greater than that at the basal end of the cell, consistent with the presence of a more extensive cytoskeleton support beneath the PM at the site of the lateral wall. The apparent viscosity of the PM was measured by pulling tethers at different rates while continuously recording the tether force, and was estimated in the range of 13-33 pN•s/μm.

AB - The mechanical characteristics of the outer hair cells (OHCs) plasma membrane was studied by forming tethers with optical tweezers. The average force to from a plasma membrane tether at the lateral wall of the OHC was about 3.5 times greater than that at the basal end of the cell, consistent with the presence of a more extensive cytoskeleton support beneath the PM at the site of the lateral wall. The apparent viscosity of the PM was measured by pulling tethers at different rates while continuously recording the tether force, and was estimated in the range of 13-33 pN•s/μm.

KW - Biomechanics

KW - Cell

KW - Electromotility

KW - Hearing

KW - Lasers

KW - Viscosity

UR - http://www.scopus.com/inward/record.url?scp=0034938381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034938381&partnerID=8YFLogxK

U2 - 10.1117/12.426765

DO - 10.1117/12.426765

M3 - Article

AN - SCOPUS:0034938381

VL - 4260

SP - 103

EP - 108

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

SN - 0277-786X

ER -