Dual CARS and SHG image acquisition scheme that combines single central fiber and multimode fiber bundle to collect and differentiate backward and forward generated photons

Sheng Weng, Xu Chen, Xiaoyun Xu, Kelvin K. Wong, Stephen T C Wong

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

In coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) imaging, backward and forward generated photons exhibit different image patterns and thus capture salient intrinsic information of tissues from different perspectives. However, they are often mixed in collection using traditional image acquisition methods and thus are hard to interpret. We developed a multimodal scheme using a single central fiber and multimode fiber bundle to simultaneously collect and differentiate images formed by these two types of photons and evaluated the scheme in an endomicroscopy prototype. The ratio of these photons collected was calculated for the characterization of tissue regions with strong or weak epiphoton generation while different image patterns of these photons at different tissue depths were revealed. This scheme provides a new approach to extract and integrate information captured by backward and forward generated photons in dual CARS/SHG imaging synergistically for biomedical applications.

Original languageEnglish (US)
Pages (from-to)2202-2218
Number of pages17
JournalBiomedical Optics Express
Volume7
Issue number6
DOIs
StatePublished - Jun 1 2016

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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