Drosophila Tempura, a novel protein prenyltransferase α subunit, regulates notch signaling via Rab1 and Rab11

Wu-Lin Charng, Shinya Yamamoto, Manish Jaiswal, Vafa Bayat, Bo Xiong, Ke Zhang, Hector Sandoval, Gabriela David, Stephen Gibbs, Hsiang-Chih Lu, Kuchuan Chen, Nikos Giagtzoglou, Hugo J Bellen

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Vesicular trafficking plays a key role in tuning the activity of Notch signaling. Here, we describe a novel and conserved Rab geranylgeranyltransferase (RabGGT)-α-like subunit that is required for Notch signaling-mediated lateral inhibition and cell fate determination of external sensory organs. This protein is encoded by tempura, and its loss affects the secretion of Scabrous and Delta, two proteins required for proper Notch signaling. We show that Tempura forms a heretofore uncharacterized RabGGT complex that geranylgeranylates Rab1 and Rab11. This geranylgeranylation is required for their proper subcellular localization. A partial dysfunction of Rab1 affects Scabrous and Delta in the secretory pathway. In addition, a partial loss Rab11 affects trafficking of Delta. In summary, Tempura functions as a new geranylgeranyltransferase that regulates the subcellular localization of Rab1 and Rab11, which in turn regulate trafficking of Scabrous and Delta, thereby affecting Notch signaling.

Original languageEnglish (US)
Pages (from-to)e1001777
JournalPLoS biology
Volume12
Issue number1
DOIs
StatePublished - Jan 2014

Keywords

  • Animals
  • Dimethylallyltranstransferase
  • Drosophila Proteins
  • Drosophila melanogaster
  • Gene Expression Regulation
  • Glycoproteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Protein Prenylation
  • Protein Processing, Post-Translational
  • Protein Subunits
  • Receptors, Notch
  • Signal Transduction
  • rab GTP-Binding Proteins
  • rab1 GTP-Binding Proteins
  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

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