Abstract
CONCLUSIONS: The Donath-Landsteiner (DL) test is a serologic test used to detect the presence of a biphasic hemolysin. This autoantibody is seen in patients with paroxysmal cold hemoglobinuria. The test relies on the characteristic cold binding of an IgG autoantibody with specificity to the P blood group antigen. This autoantibody causes complement-mediated red blood cell (RBC) lysis when warmed to body temperature. In this review, we describe the various methods for performing the DL test-namely a direct test, an indirect test, an indirect test with modifications such as the use of enzyme-treated RBCs and two stages, and an indirect antiglobulin DL test-and highlight the advantages and disadvantages of each. Our focus is on the indirect testing method as it is most commonly used in blood bank laboratories.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3-6 |
| Number of pages | 4 |
| Journal | Immunohematology |
| Volume | 35 |
| Issue number | 1 |
| State | Published - Jan 1 2019 |
Keywords
- Autoantibodies
- Blood Banks
- Cold Temperature
- Hemoglobinuria, Paroxysmal
- Humans
- Serologic Tests
ASJC Scopus subject areas
- Immunology and Allergy
- Hematology