Domain structure of the glucocorticoid receptor protein.

J. Carlstedt-Duke; P. E. Strömstedt; O. Wrange; T. Bergman; J. A. Gustafsson; H. Jörnvall

doi:10.1073/pnas.84.13.4437

Domain structure of the glucocorticoid receptor protein.

J. Carlstedt-Duke, P. E. Strömstedt, O. Wrange, T. Bergman, J. A. Gustafsson, H. Jörnvall

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Abstract

The purified rat liver glucocorticoid receptor protein was analyzed by limited proteolysis and amino acid sequence determination. The NH2 terminus appears to be blocked. The steroid-binding domain, defined by a unique tryptic cleavage site, corresponds to the COOH-terminal part of the protein with the domain border in the region of residue 518. The DNA-binding domain, defined by a region with chymotryptic cleavage sites, is immediately adjacent to the steroid-binding domain and reflects another domain border in the region of residues 410-414. The results described at the protein level in this report confirm functional data previously obtained by mutations at the genetic level.

Original language	English (US)
Pages (from-to)	4437-4440
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	84
Issue number	13
DOIs	https://doi.org/10.1073/pnas.84.13.4437
State	Published - Jul 1987

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title = "Domain structure of the glucocorticoid receptor protein.",

abstract = "The purified rat liver glucocorticoid receptor protein was analyzed by limited proteolysis and amino acid sequence determination. The NH2 terminus appears to be blocked. The steroid-binding domain, defined by a unique tryptic cleavage site, corresponds to the COOH-terminal part of the protein with the domain border in the region of residue 518. The DNA-binding domain, defined by a region with chymotryptic cleavage sites, is immediately adjacent to the steroid-binding domain and reflects another domain border in the region of residues 410-414. The results described at the protein level in this report confirm functional data previously obtained by mutations at the genetic level.",

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AU - Carlstedt-Duke, J.

AU - Strömstedt, P. E.

AU - Wrange, O.

AU - Bergman, T.

AU - Gustafsson, J. A.

AU - Jörnvall, H.

PY - 1987/7

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Domain structure of the glucocorticoid receptor protein.

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