Domain structure of the glucocorticoid receptor protein.

J. Carlstedt-Duke, P. E. Strömstedt, O. Wrange, T. Bergman, J. A. Gustafsson, H. Jörnvall

    Research output: Contribution to journalArticlepeer-review

    92 Scopus citations

    Abstract

    The purified rat liver glucocorticoid receptor protein was analyzed by limited proteolysis and amino acid sequence determination. The NH2 terminus appears to be blocked. The steroid-binding domain, defined by a unique tryptic cleavage site, corresponds to the COOH-terminal part of the protein with the domain border in the region of residue 518. The DNA-binding domain, defined by a region with chymotryptic cleavage sites, is immediately adjacent to the steroid-binding domain and reflects another domain border in the region of residues 410-414. The results described at the protein level in this report confirm functional data previously obtained by mutations at the genetic level.

    Original languageEnglish (US)
    Pages (from-to)4437-4440
    Number of pages4
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume84
    Issue number13
    DOIs
    StatePublished - Jul 1987

    ASJC Scopus subject areas

    • General

    Fingerprint

    Dive into the research topics of 'Domain structure of the glucocorticoid receptor protein.'. Together they form a unique fingerprint.

    Cite this