DNA methylation-associated colonic mucosal immune and defense responses in treatmentnaïve pediatric ulcerative colitis

R. Alan Harris, Dorottya Nagy-Szakal, Sabina A.V. Mir, Eibe Frank, Reka Szigeti, Jess L. Kaplan, Jiri Bronsky, Antone Opekun, George D. Ferry, Harland Winter, Richard Kellermayer

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


Inflammatory bowel diseases (IBD) are emerging globally, indicating that environmental factors may be important in their pathogenesis. Colonic mucosal epigenetic changes, such as DNA methylation, can occur in response to the environment and have been implicated in IBD pathology. However, mucosal DNA methylation has not been examined in treatment-naïve patients. We studied DNA methylation in untreated, left sided colonic biopsy specimens using the Infinium HumanMethylation450 Bead Chip array. We analyzed 22 control (C) patients, 15 untreated crohn's disease (CD) patients, and 9 untreated ulcerative colitis (UC) patients from two cohorts. Samples obtained at the time of clinical remission from two of the treatment-naïve UC patients were also included into the analysis. UC-specific gene expression was interrogated in a subset of adjacent samples (5 c and 5 UC) using the Affymetrix GeneChip PrimeView Human Gene expression arrays. Only treatment-naïve UC separated from control. One-hundred-and-20 genes with significant expression change in UC(> 2-fold, P < 0.05) were associated with differentially methylated regions (DMRs). Epigenetically associated gene expression changes (including gene expression changes in the IFITM1, ITGB2, S100A9, SLPI, SAA1, and STAT3 genes) were linked to colonic mucosal immune and defense responses. These findings underscore the relationship between epigenetic changes and inflammation in pediatric treatment-naïve UC and may have potential etiologic, diagnostic, and therapeutic relevance for IBD.

Original languageEnglish (US)
Pages (from-to)1131-1137
Number of pages7
Issue number8
StatePublished - Jun 17 2014


  • DNA expression
  • DNA methylation
  • DNA methylome
  • Epigenetics
  • Pediatric inflammatory bowel disease
  • Treatment-naïve
  • Ulcerative colitis

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research


Dive into the research topics of 'DNA methylation-associated colonic mucosal immune and defense responses in treatmentnaïve pediatric ulcerative colitis'. Together they form a unique fingerprint.

Cite this