DNA binding of glucocorticoid receptor protein a fusion proteins expressed in E. coli

Constanze Bonifer, Karin Dahlman, Per Erik Strömstedt, Jan Ingmar Flock, Jan Åke Gustafsson

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

In an effort to obtain large quantities of glucocorticoid receptor (GR) protein for functional and structural studies, several truncated versions of the human glucocorticoid receptor (hGR) have been expressed in E. coli as C-terminal fusion proteins with protein A. The amount of expressed protein was between 5 and 25 mg/l in the culture. South-Western blotting was initially used to demonstrate the DNA binding capacity of fusion proteins containing the DNA binding domain of GR. The hybrid proteins were highly enriched in the insoluble fraction after cell lysis. For further purification and characterization the fusion proteins were solubilized in 8 M urea. The concentration of denaturing agent was reduced by dilution and the fusion proteins were allowed to refold. The renatured GR protein A fusion proteins bound to DNA in a nitrocellulose filter binding assay. We also show that it is possible to purify the renatured fusion protein to apparent homogeneity using a single Chromatographic step on DNA-cellulose.

Original languageEnglish (US)
Pages (from-to)5-11
Number of pages7
JournalJournal of Steroid Biochemistry
Volume32
Issue number1 PART 1
DOIs
StatePublished - Jan 1989

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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