Diurnal variations in excretion of corticosteroid metabolites in bile from male and female rats

René Jean Begue, Jan Åke Gustafsson, Sven A. Gustafsson

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10 Scopus citations

Abstract

The excretion of corticosteroids in bile has been studied in female and male rats. The total daily excretion of corticosteroids in female rats was 738 ± 169 ms (3α,11β,15α,21-tetrahydroxy-5α-pregnan-20-one monosulphate: 300 ± 16 μg; 3α,11β,15α,21-tetrahydroxy-5α-pregnan-20-one disulphate: 158 ± 51 μg; 3α,11β,21-trihydroxy-5α-pregnan-20-one disulphate: 146 ± 49 μg; and 3β,11β,21-trihydroxy-5α-pregnan-20-one disulphate: 134 ± 38μg) and in male rats the corresponding figure was 773 ± 97 μg (3β,11β,21-trihydroxy-5α-pregnan-20-one disulphate: 682 ± 72 μg; and 5α-pregnane-3β,11β,20β,21-tetrol: 91 ± 25μg). The excretion of corticosteroids was cyclic in both female and male rats with the maximum excretion between 16.00 and 22.00 h and the minimum excretion between 04.00 and 10.00 h. The daily rhythm of corticosteroid excretion was disturbed for at least two days by operative manipulations in connection with bile duct cannulation, by intraperitoneal administration of saline and by intramuscular administration of dimethylsulphoxide.

Original languageEnglish (US)
Pages (from-to)393-400
Number of pages8
JournalJournal of Steroid Biochemistry
Volume4
Issue number4
DOIs
StatePublished - Jul 1973

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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