TY - JOUR
T1 - Distribution of streptococcal inhibitor of complement variants in pharyngitis and invasive isolates in an epidemic of serotype M1 group A Streptococcus infection
AU - Hoe, Nancy P.
AU - Vuopio-Varkila, Jaana
AU - Vaara, Martti
AU - Grigsby, Diana
AU - De Lorenzo, David
AU - Fu, Yun Xin
AU - Dou, Shu Jun
AU - Pan, Xi
AU - Nakashima, Kazumitsu
AU - Musser, James M.
N1 - Funding Information:
Received 26 September 2000; revised 7 November 2000; electronically published 17 January 2001. Presented in part: 100th general meeting of the American Society for Microbiology, Los Angeles, California, 21–25 May 2000 (abstract D-3). Financial support: US Public Health Service grant GM-50428 (to Y.-X.F.). a Present affiliations: Boston University School of Public Health, Boston, Massachusetts (D.G.); Department of Respiratory Diseases, Chubu National Hospital, Aichi, Japan (K.N.). Reprints or correspondence: Dr. James M. Musser, Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 S. 4th St., Hamilton, MT 59840 ([email protected]).
PY - 2001/2/15
Y1 - 2001/2/15
N2 - Streptococcal inhibitor of complement (Sic) is a highly polymorphic extracellular protein made predominantly by serotype M1 group A Streptococcus (GAS). New variants of the Sic protein frequently appear in M1 epidemics as a result of positive natural selection. To gain further understanding of the molecular basis of M1 epidemics, the sic gene was sequenced from 471 pharyngitis and 127 pyogenic and blood isolates recovered from 598 patients living in metropolitan Helsinki, Finland, during a 37-month population-based surveillance study. Most M1 GAS subclones recovered from pyogenic infections and blood were abundantly represented in the pool of subclones causing pharyngitis. Alleles shared among the pharyngitis, pyogenic, and blood samples were identified in throat isolates a mean of 9.8 months before their recovery from pyogenic infections and blood, which indicates that selection of most sic variants occurs on mucosal surfaces. In contrast, no variation was identified in the emm and covR/covS genes.
AB - Streptococcal inhibitor of complement (Sic) is a highly polymorphic extracellular protein made predominantly by serotype M1 group A Streptococcus (GAS). New variants of the Sic protein frequently appear in M1 epidemics as a result of positive natural selection. To gain further understanding of the molecular basis of M1 epidemics, the sic gene was sequenced from 471 pharyngitis and 127 pyogenic and blood isolates recovered from 598 patients living in metropolitan Helsinki, Finland, during a 37-month population-based surveillance study. Most M1 GAS subclones recovered from pyogenic infections and blood were abundantly represented in the pool of subclones causing pharyngitis. Alleles shared among the pharyngitis, pyogenic, and blood samples were identified in throat isolates a mean of 9.8 months before their recovery from pyogenic infections and blood, which indicates that selection of most sic variants occurs on mucosal surfaces. In contrast, no variation was identified in the emm and covR/covS genes.
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U2 - 10.1086/318543
DO - 10.1086/318543
M3 - Article
C2 - 11170990
AN - SCOPUS:0035865911
SN - 0022-1899
VL - 183
SP - 633
EP - 639
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -