TY - JOUR
T1 - Distinct signalling pathways regulate sprouting angiogenesis from the dorsal aorta and the axial vein
AU - Wiley, David M.
AU - Kim, Jun Dae
AU - Hao, Jijun
AU - Hong, Charles C.
AU - Bautch, Victoria L.
AU - Jin, Suk Won
N1 - Funding Information:
This study was supported by grants from the NIH to S-W.J. (HL090960) and V.L.B. (HL43174 and HL86564), and the UNC Integrative Vascular Biology Training Grant (T32HL69768) and an American Heart Association Pre-doctoral Fellowship to D.M.W.
PY - 2011/6
Y1 - 2011/6
N2 - Angiogenesis, the formation of new blood vessels from pre-existing vessels, is critical to most physiological processes and many pathological conditions. During zebrafish development, angiogenesis expands the axial vessels into a complex vascular network that is necessary for efficient oxygen delivery. Although the dorsal aorta and the axial vein are spatially juxtaposed, the initial angiogenic sprouts from these vessels extend in opposite directions, indicating that distinct cues may regulate angiogenesis of the axial vessels. We found that angiogenic sprouts from the dorsal aorta are dependent on vascular endothelial growth factor A (Vegf-A) signalling, and do not respond to bone morphogenetic protein (Bmp) signals. In contrast, sprouts from the axial vein are regulated by Bmp signalling independently of Vegf-A signals, indicating that Bmp is a vein-specific angiogenic cue during early vascular development. Our results support a paradigm whereby different signals regulate distinct programmes of sprouting angiogenesis from the axial vein and dorsal aorta, and indicate that signalling heterogeneity contributes to the complexity of vascular networks.
AB - Angiogenesis, the formation of new blood vessels from pre-existing vessels, is critical to most physiological processes and many pathological conditions. During zebrafish development, angiogenesis expands the axial vessels into a complex vascular network that is necessary for efficient oxygen delivery. Although the dorsal aorta and the axial vein are spatially juxtaposed, the initial angiogenic sprouts from these vessels extend in opposite directions, indicating that distinct cues may regulate angiogenesis of the axial vessels. We found that angiogenic sprouts from the dorsal aorta are dependent on vascular endothelial growth factor A (Vegf-A) signalling, and do not respond to bone morphogenetic protein (Bmp) signals. In contrast, sprouts from the axial vein are regulated by Bmp signalling independently of Vegf-A signals, indicating that Bmp is a vein-specific angiogenic cue during early vascular development. Our results support a paradigm whereby different signals regulate distinct programmes of sprouting angiogenesis from the axial vein and dorsal aorta, and indicate that signalling heterogeneity contributes to the complexity of vascular networks.
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U2 - 10.1038/ncb2232
DO - 10.1038/ncb2232
M3 - Article
C2 - 21572418
AN - SCOPUS:79957910125
VL - 13
SP - 686
EP - 693
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6
ER -