TY - JOUR
T1 - Distinct Genetic Diversity of Carbapenem-Resistant Acinetobacter baumannii from Colombian Hospitals
AU - Correa, Adriana
AU - Del Campo, Rosa
AU - Escandón-Vargas, Kevin
AU - Perenguez, Marcela
AU - Rodríguez-Banõs, Mercedes
AU - Hernández-Gómez, Cristhian
AU - Pallares, Christian
AU - Perez, Federico
AU - Arias, Cesar A.
AU - Cantón, Rafael
AU - Villegas, Mariá V.
N1 - Funding Information:
This work was supported by Merck Sharp & Dohme, Janssen-Cilag, Pfizer, AstraZeneca, Merck, Novartis, Amarey Nova Medical, Merck, and bioMérieux Colombia, which helped funding the Colombian Nosocomial Resistance Study Group.
Funding Information:
A.C. has received speaker honoraria from Merck Sharp & Dohme and bioMérieux. C.A.A. has received grant support form Pfizer, Forest Pharmaceuticals, and Theravance, Inc., has served on the speaker bureaus of Cubist, Forest Pharmaceuticals, Pfizer, Novartis, and AstraZeneca, and has performed consulting activities for Cubist, Bayer, and As-traZeneca. He is supported by grant K24-AI114818. R.C.’s research at the Servicio de Microbiología del Hospital Uni-versitario Ramón y Cajal was funded by the European Commission (grants R-GNOSIS-FP7-HEALTH-F3-2011-282512 and FP7-HEALTH-F3-2013-MON4STRAT-602906-2). M.V.V. has received consulting fees and research grants from Merck Sharp & Dohme, Pfizer, Janssen-Cilag, Novartis, Merck, and AstraZeneca. The other authors declare no competing interests.
Publisher Copyright:
© 2018 Mary Ann Liebert, Inc.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The global success of multidrug-resistant Acinetobacter baumannii has been associated with the dissemination of a high-risk clone designated clonal complex (CC) 92B (Bartual scheme)/CC2P (Pasteur scheme), which is the most frequent genetic lineage in European, Asian, and North American carbapenem-resistant Acinetobacter isolates. In these isolates, carbapenem resistance is mainly mediated by β-lactamases encoded by blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, and/or blaOXA-58-like genes. In this study, we characterized the population genetics of 121 carbapenem-resistant A. baumannii complex isolates recovered from 14 hospitals in seven cities in Colombia (2008-2010). Multiplex PCR was used to detect blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, and blaOXA-58-like genes. Molecular typing was performed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). PCR showed that 118 (97.5%) of the isolates were positive for both blaOXA-23-like and blaOXA-51-like genes, and three other isolates were only positive for blaOXA-51-like. PFGE identified 18 different pulsotypes, while MLST identified 11 different sequence types (STs), seven of which had not been previously described in Acinetobacter. None of the STs found in this study was associated with CC92B/CC2P. The most widespread STs in our isolates belonged to ST636 and their single-locus variants ST121/ST124/ST634 (CC636B) followed by STs belonging to CC110B. Our observations suggest a wide distribution of diverse A. baumannii complex clones containing blaOXA-23-like in Colombian hospitals (especially CC636B and CC110B) that differ from the high-risk clones commonly found in other regions of the world, indicating a distinct molecular epidemiology of carbapenem-resistant Acinetobacter spp. in Colombia.
AB - The global success of multidrug-resistant Acinetobacter baumannii has been associated with the dissemination of a high-risk clone designated clonal complex (CC) 92B (Bartual scheme)/CC2P (Pasteur scheme), which is the most frequent genetic lineage in European, Asian, and North American carbapenem-resistant Acinetobacter isolates. In these isolates, carbapenem resistance is mainly mediated by β-lactamases encoded by blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, and/or blaOXA-58-like genes. In this study, we characterized the population genetics of 121 carbapenem-resistant A. baumannii complex isolates recovered from 14 hospitals in seven cities in Colombia (2008-2010). Multiplex PCR was used to detect blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, and blaOXA-58-like genes. Molecular typing was performed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). PCR showed that 118 (97.5%) of the isolates were positive for both blaOXA-23-like and blaOXA-51-like genes, and three other isolates were only positive for blaOXA-51-like. PFGE identified 18 different pulsotypes, while MLST identified 11 different sequence types (STs), seven of which had not been previously described in Acinetobacter. None of the STs found in this study was associated with CC92B/CC2P. The most widespread STs in our isolates belonged to ST636 and their single-locus variants ST121/ST124/ST634 (CC636B) followed by STs belonging to CC110B. Our observations suggest a wide distribution of diverse A. baumannii complex clones containing blaOXA-23-like in Colombian hospitals (especially CC636B and CC110B) that differ from the high-risk clones commonly found in other regions of the world, indicating a distinct molecular epidemiology of carbapenem-resistant Acinetobacter spp. in Colombia.
KW - Acinetobacter baumannii
KW - OXA enzymes
KW - antibiotic resistance
KW - carbapenemase
KW - multilocus sequence typing
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U2 - 10.1089/mdr.2016.0190
DO - 10.1089/mdr.2016.0190
M3 - Article
C2 - 28570118
AN - SCOPUS:85040543528
SN - 1076-6294
VL - 24
SP - 48
EP - 54
JO - Microbial Drug Resistance
JF - Microbial Drug Resistance
IS - 1
ER -