TY - JOUR
T1 - Dissociated single cells from the inner ear
T2 - Strial cells
AU - Lim, David J.
PY - 1985
Y1 - 1985
N2 - Using the enzyme dissociation technique, the authors successfully isolated strial marginal cells and intermediate cells from guinea pig and chinchilla cochleas. Dissociated cells were maintained for in vitro obsevation under the light microscope for up to 2 hours without gross evidence of cell damage. Such single cells were successfully prepared for scanning or transmission electron microscopic observation, which showed excellent cell shape and preservation of cell organelles. However, there was evidence of increased vesiculation in the dissociated marginal cells, which is interpreted to be evidence of increased pinocytotic activity.
AB - Using the enzyme dissociation technique, the authors successfully isolated strial marginal cells and intermediate cells from guinea pig and chinchilla cochleas. Dissociated cells were maintained for in vitro obsevation under the light microscope for up to 2 hours without gross evidence of cell damage. Such single cells were successfully prepared for scanning or transmission electron microscopic observation, which showed excellent cell shape and preservation of cell organelles. However, there was evidence of increased vesiculation in the dissociated marginal cells, which is interpreted to be evidence of increased pinocytotic activity.
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U2 - 10.1016/S0196-0709(85)80078-8
DO - 10.1016/S0196-0709(85)80078-8
M3 - Article
C2 - 2990247
AN - SCOPUS:0021840697
SN - 0196-0709
VL - 6
SP - 153
EP - 162
JO - American Journal of Otolaryngology - Head and Neck Medicine and Surgery
JF - American Journal of Otolaryngology - Head and Neck Medicine and Surgery
IS - 3
ER -