Direct antimicrobial drug susceptibility testing of Mycobacterium tuberculosis by the radiometric method

Joseph P. Libonati, Charles E. Stager, James R. Davis, Salman H. Siddiqi

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Direct-drug-susceptibility tests were performed on clinical specimens positive for acid-fast bacilli by either Ziehl-Neelsen or fluorochrome staining. The results of conventional agar dilution (Vestal, 1975) and a modified radiometric (BACTEC) method were compared. A total of 580 smear-positive specimens were tested by the BACTEC method at three separate sites. Three hundred and seventy-seven of these were culture positive for M. tuberculosis, and 343 (91%) yielded acceptable direct-susceptibility-test results. We used the conventional method to determine that 343 of 519 smear-positive specimens were culture positive for M. tuberculosis, and 212 (62%) produced acceptable results within 3 wks. Conventional results were reported in 3-4 wks, while the time required to obtain results with the BACTEC method ranged from 5 to 21 days (average 11.5 days). Results indicate that the radiometric method provides reportable results more frequently with time savings as compared to the conventional method.

Original languageEnglish (US)
Pages (from-to)41-48
Number of pages8
JournalDiagnostic Microbiology and Infectious Disease
Volume10
Issue number1
DOIs
StatePublished - May 1988

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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