TY - JOUR
T1 - Dilute suppressor dsu acts semidominantly to suppress the coat color phenotype of a deletion mutation, d(l20J), of the murine dilute locus
AU - Moore, K. J.
AU - Seperack, P. K.
AU - Strobel, M. C.
AU - Swing, D. A.
AU - Copeland, N. G.
AU - Jenkins, N. A.
PY - 1988
Y1 - 1988
N2 - The murine dilute suppressor (dsu) gene is the only unlinked trans-acting suppressor identified in mammals. dsu, which was originally reported to be recessive, was recognized by its ability to suppress the coat color phenotype of a retroviral insertion mutation, d(v), of the murine dilute (d) locus. This insertion mutation resulted from the integration of an ecotropic murine leukemia virus into noncoding sequences of the dilute gene. Therefore, dsu may act like other allele-specific recessive suppressors identified in Drosophila melanogaster and yeast that suppress mutations induced by retrotransposon insertions. To investigate this possibility, we have examined whether dsu could suppress a spontaneously arising allele of d, d(l20J), which is shown here to result from a 3.5-kilobase deletion. These studies indicate that dsu does not function like other eukaryotic suppressor genes that suppress retrotransposon-induced mutations. We also show that dsu is not, as originally reported, a recessive gene but is semidominantly inherited. Collectively, these results allow us to propose a mechanism for the suppressor activity of dsu.
AB - The murine dilute suppressor (dsu) gene is the only unlinked trans-acting suppressor identified in mammals. dsu, which was originally reported to be recessive, was recognized by its ability to suppress the coat color phenotype of a retroviral insertion mutation, d(v), of the murine dilute (d) locus. This insertion mutation resulted from the integration of an ecotropic murine leukemia virus into noncoding sequences of the dilute gene. Therefore, dsu may act like other allele-specific recessive suppressors identified in Drosophila melanogaster and yeast that suppress mutations induced by retrotransposon insertions. To investigate this possibility, we have examined whether dsu could suppress a spontaneously arising allele of d, d(l20J), which is shown here to result from a 3.5-kilobase deletion. These studies indicate that dsu does not function like other eukaryotic suppressor genes that suppress retrotransposon-induced mutations. We also show that dsu is not, as originally reported, a recessive gene but is semidominantly inherited. Collectively, these results allow us to propose a mechanism for the suppressor activity of dsu.
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U2 - 10.1073/pnas.85.21.8131
DO - 10.1073/pnas.85.21.8131
M3 - Article
C2 - 3141922
AN - SCOPUS:0008329030
SN - 0027-8424
VL - 85
SP - 8131
EP - 8135
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 21
ER -