Differentiation of neuroepithelia from human embryonic stem cells

Xiaofeng Xia, Su Chun Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

17 Scopus citations

Abstract

We describe the method for efficiently differentiating human embryonic stem cells to neuroepithelial cells in a chemically defined condition. The protocol was established based on the fundamental principle of in vivo neuroectodermal development. The temporal course, morphological transformation, and shift in gene expression of our neuroepithelial differentiation closely resemble those occur during in vivo development. In particular, the primitive neuroepithelial cells generated by this protocol can be further induced into neuronal and glial cells with forebrain, mid/hind brain, and spinal cord identities and targeted transmitter phenotypes.

Original languageEnglish (US)
Title of host publicationNeural Cell Transplantation
Subtitle of host publicationMethods and Protocols
EditorsNeil Scolding, David Gordon
Pages51-58
Number of pages8
DOIs
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
Volume549
ISSN (Print)1064-3745

Keywords

  • Chemically defined condition
  • Embryoid body
  • Human embryonic stem cells
  • Neuroepithelia
  • Neuronal differentiation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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