Differentiation of neuroepithelia from human embryonic stem cells

Xiaofeng Xia; Su Chun Zhang

doi:10.1007/978-1-60327-931-4_4

Differentiation of neuroepithelia from human embryonic stem cells

Xiaofeng Xia, Su Chun Zhang

Academic Institute

Research output: Chapter in Book/Report/Conference proceeding › Chapter

23 Scopus citations

Abstract

We describe the method for efficiently differentiating human embryonic stem cells to neuroepithelial cells in a chemically defined condition. The protocol was established based on the fundamental principle of in vivo neuroectodermal development. The temporal course, morphological transformation, and shift in gene expression of our neuroepithelial differentiation closely resemble those occur during in vivo development. In particular, the primitive neuroepithelial cells generated by this protocol can be further induced into neuronal and glial cells with forebrain, mid/hind brain, and spinal cord identities and targeted transmitter phenotypes.

Original language	English (US)
Title of host publication	Neural Cell Transplantation
Subtitle of host publication	Methods and Protocols
Editors	Neil Scolding, David Gordon
Pages	51-58
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-60327-931-4_4
State	Published - 2009

Publication series

Name	Methods in Molecular Biology
Volume	549
ISSN (Print)	1064-3745

Keywords

Chemically defined condition
Embryoid body
Human embryonic stem cells
Neuroepithelia
Neuronal differentiation

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-60327-931-4_4

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KW - Neuroepithelia

KW - Neuronal differentiation

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Differentiation of neuroepithelia from human embryonic stem cells

Abstract

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Keywords

ASJC Scopus subject areas

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