Differentiating between Burkitt lymphoma and CD10+ diffuse large B-cell lymphoma: The role of commonly used flow cytometry cell markers and the application of a multiparameter scoring system

Paul McGowan, Nicole Nelles, Jana Wimmer, Dawn Williams, Jianguo Wen, Marilyn Li, April A. Ewton, Choladda Curry, Youli Zu, Andrea Sheehan, Chung Che Chang

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The goal of this study was to evaluate routine flow cytometric (FC) immunophenotypic markers in differentiating between Burkitt lymphoma (BL) and CD10+ diffuse large B-cell lymphoma (DLBCL). We performed retrospective analysis of FC data from 55 patients. We evaluated 9 FC parameters: forward and side scatter (FSC and SSC); mean fluorescent intensity (MFI) for CD20, CD10, CD38, CD79b, CD43, and CD71; and the percentage of neoplastic cells positive for CD71 (%CD71). The FSC; MFIs of CD10, CD43, CD79b, and CD71; and %CD71 cells were significantly different between BL and CD10+ DLBCL (P < .05; Student t test). A 5-point scoring system (FSC, %CD71, and MFIs of CD43, CD79b, and CD71) was devised, and 6 (60%) of 10 BLs scored 3 or greater and 1 (10%) of 10 CD10+ DLBCLs scored 3 (P = .04; χ 2). Our findings indicate that routine FC parameters can aid in differentiating BL from CD10+ DLBCL. Copyright

Original languageEnglish (US)
Pages (from-to)665-670
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume137
Issue number4
DOIs
StatePublished - Apr 2012

Keywords

  • Burkitt lymphoma
  • CD10+ diffuse large B-cell lymphoma
  • Flow cytometry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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