TY - JOUR
T1 - Differentiating between Burkitt lymphoma and CD10+ diffuse large B-cell lymphoma
T2 - The role of commonly used flow cytometry cell markers and the application of a multiparameter scoring system
AU - McGowan, Paul
AU - Nelles, Nicole
AU - Wimmer, Jana
AU - Williams, Dawn
AU - Wen, Jianguo
AU - Li, Marilyn
AU - Ewton, April A.
AU - Curry, Choladda
AU - Zu, Youli
AU - Sheehan, Andrea
AU - Chang, Chung Che
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/4
Y1 - 2012/4
N2 - The goal of this study was to evaluate routine flow cytometric (FC) immunophenotypic markers in differentiating between Burkitt lymphoma (BL) and CD10+ diffuse large B-cell lymphoma (DLBCL). We performed retrospective analysis of FC data from 55 patients. We evaluated 9 FC parameters: forward and side scatter (FSC and SSC); mean fluorescent intensity (MFI) for CD20, CD10, CD38, CD79b, CD43, and CD71; and the percentage of neoplastic cells positive for CD71 (%CD71). The FSC; MFIs of CD10, CD43, CD79b, and CD71; and %CD71 cells were significantly different between BL and CD10+ DLBCL (P < .05; Student t test). A 5-point scoring system (FSC, %CD71, and MFIs of CD43, CD79b, and CD71) was devised, and 6 (60%) of 10 BLs scored 3 or greater and 1 (10%) of 10 CD10+ DLBCLs scored 3 (P = .04; χ 2). Our findings indicate that routine FC parameters can aid in differentiating BL from CD10+ DLBCL. Copyright
AB - The goal of this study was to evaluate routine flow cytometric (FC) immunophenotypic markers in differentiating between Burkitt lymphoma (BL) and CD10+ diffuse large B-cell lymphoma (DLBCL). We performed retrospective analysis of FC data from 55 patients. We evaluated 9 FC parameters: forward and side scatter (FSC and SSC); mean fluorescent intensity (MFI) for CD20, CD10, CD38, CD79b, CD43, and CD71; and the percentage of neoplastic cells positive for CD71 (%CD71). The FSC; MFIs of CD10, CD43, CD79b, and CD71; and %CD71 cells were significantly different between BL and CD10+ DLBCL (P < .05; Student t test). A 5-point scoring system (FSC, %CD71, and MFIs of CD43, CD79b, and CD71) was devised, and 6 (60%) of 10 BLs scored 3 or greater and 1 (10%) of 10 CD10+ DLBCLs scored 3 (P = .04; χ 2). Our findings indicate that routine FC parameters can aid in differentiating BL from CD10+ DLBCL. Copyright
KW - Burkitt lymphoma
KW - CD10+ diffuse large B-cell lymphoma
KW - Flow cytometry
UR - http://www.scopus.com/inward/record.url?scp=84860595836&partnerID=8YFLogxK
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U2 - 10.1309/AJCP3FEPX5BEEKGX
DO - 10.1309/AJCP3FEPX5BEEKGX
M3 - Article
C2 - 22431545
AN - SCOPUS:84860595836
SN - 0002-9173
VL - 137
SP - 665
EP - 670
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 4
ER -