Abstract
OBJECTIVE: To determine whether the miRNA expression profile in peripheral blood mononuclear cells (PBMCs) differs between liver transplant recipients with long-term stable survival and those with acute rejection.
METHODS: Twenty-nine liver transplant recipients with long-term stable survival (STA) group, 10 recipients with acute rejection (RJ group), and 17 healthy subjects (control group) were recruited for genome-wide microarray analysis of miRNA expressions in the PBMCs. The differentially expressed miRNAs among the 3 groups were validated by real-time PCR, and the targets of these miRNAs were predicted.
RESULTS: Compared with the RJ group, the STA group showed down-regulation of 13 miRNAs in the PBMCs. Of these down-regulated miRNAs, miRNA-18b, miRNA-340 and miRNA-106b were validated by real-time PCR, and the latter two miRNAs were predicted to target the TGF-β pathway.
CONCLUSIONS: The differentially expressed miRNAs in liver transplant recipients with long-term stable survival, namely miRNA-18b, miRNA-340 and miRNA-106b, can be potential clinical biomarkers to predict the outcomes of liver transplantation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1557-1563 |
| Number of pages | 7 |
| Journal | Nan fang yi ke da xue xue bao = Journal of Southern Medical University |
| Volume | 35 |
| Issue number | 11 |
| State | Published - Nov 1 2015 |
ASJC Scopus subject areas
- Medicine(all)