Differential expressions of microRNAs and their predicted targets in liver transplant recipients with long-term stable survival

Kebo Zhong, Peng Zhang, Xiaoshun He, Xiaofeng Zhu

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

OBJECTIVE: To determine whether the miRNA expression profile in peripheral blood mononuclear cells (PBMCs) differs between liver transplant recipients with long-term stable survival and those with acute rejection.

METHODS: Twenty-nine liver transplant recipients with long-term stable survival (STA) group, 10 recipients with acute rejection (RJ group), and 17 healthy subjects (control group) were recruited for genome-wide microarray analysis of miRNA expressions in the PBMCs. The differentially expressed miRNAs among the 3 groups were validated by real-time PCR, and the targets of these miRNAs were predicted.

RESULTS: Compared with the RJ group, the STA group showed down-regulation of 13 miRNAs in the PBMCs. Of these down-regulated miRNAs, miRNA-18b, miRNA-340 and miRNA-106b were validated by real-time PCR, and the latter two miRNAs were predicted to target the TGF-β pathway.

CONCLUSIONS: The differentially expressed miRNAs in liver transplant recipients with long-term stable survival, namely miRNA-18b, miRNA-340 and miRNA-106b, can be potential clinical biomarkers to predict the outcomes of liver transplantation.

Original languageEnglish (US)
Pages (from-to)1557-1563
Number of pages7
JournalNan fang yi ke da xue xue bao = Journal of Southern Medical University
Volume35
Issue number11
StatePublished - Nov 1 2015

ASJC Scopus subject areas

  • Medicine(all)

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