Differential expression of estrogen receptors α and β mRNA during differentiation of human osteoblast SV-HFO cells

Janine Arts, George G.J.M. Kuiper, Josine M.M.F. Janssen, Jan Åke Gustafsson, Clemens W.G.M. Löwik, Huibert A.P. Pols, Johannes P.T.M. Van Leeuwen

Research output: Contribution to journalArticle

285 Scopus citations

Abstract

Estrogens have been shown to be essential for maintaining a sufficiently high bone mineral density and ERα expression has been demonstrated in bone cells. Recently, a novel estrogen receptor, estrogen receptor β (ERβ) has been identified. Here we demonstrate that also ERβ is expressed in human osteoblasts, and that ERα and ERβ are differentially expressed during human osteoblast differentiation. ERβ mRNA expression increased gradually during osteoblast culture, resulting in an average increase of 9.9±5.3 fold (mean±S.D., n=3) at day 21 (mineralization phase) as compared to day 6 (proliferation phase). In contrast, ERα mRNA expression levels increased only slightly until day 10 (2.3±1.7 fold) and than remained constant. The observed differential regulation of ERα and β is suggestive for an additional functional role of ERβ to ERα in bone metabolism.

Original languageEnglish (US)
Pages (from-to)5067-5070
Number of pages4
JournalEndocrinology
Volume138
Issue number11
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Endocrinology

Fingerprint Dive into the research topics of 'Differential expression of estrogen receptors α and β mRNA during differentiation of human osteoblast SV-HFO cells'. Together they form a unique fingerprint.

Cite this