Differential expression of ccrA in methicillin-resistant Staphylococcus aureus strains carrying staphylococcal cassette chromosome mec type II and IVa elements

Paul G. Higgins, Adriana E. Rosato, Harald Seifert, Gordon L. Archer, Hilmar Wisplinghoff

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Excision of staphylococcal cassette chromosome mec (SCCmec) is mediated through the ccrA- and -B-encoded recombinases. We investigated the effects of different antimicrobial agents on ccrA expression by using a ccrA::lacZ fusion and reverse transcription-PCR with methicillin (meticillin)-resistant Staphylococcus aureus strains MW2 (SCCmec IVa) and N315 (SCCmec II). Upregulation of ccrA was observed upon exposure to β-lactam antibiotics. Vancomycin increased ccrA expression in MW2 but had no effect on N315. Vancomycin may contribute to the transfer of SCCmec IVa but have no effect in SCCmec II.

Original languageEnglish (US)
Pages (from-to)4556-4558
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume53
Issue number10
DOIs
StatePublished - Oct 2009

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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