Differential cytotoxicity of metal oxide nanoparticles

J. Chen, J. Zhu, H. H. Cho, K. Cui, F. Li, X. Zhou, J. T. Rogers, S. T.C. Wong, X. Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Concerns about potential health hazard of nanomaterials are growing. To determine the potential toxicity of metal oxide nanoparticles, human SH-SY5Y neuroblastoma and H4 neuroglioma cells were exposed to Fe2O 3, CuO, and ZnO nanoparticles and their metal ion counterparts (Fe3+, Cu2+, and Zn2+) at a concentration range of 0.01-100 μM for 48 hours, under the cell culture conditions: 95% O 2, 5% CO2, 85% humidity, 37°C. Their ensemble cell viability was determined by MTS cell proliferation assays. A live/dead cell assay was also performed, and cellular images were acquired by a high-content fluorescence microscope and quantified by a novel computerized image analysis protocol. Our data indicated that exposure of these nanoparticles induced differential toxic effects in both SHSY5Y and H4 cells, and the cells had dose-dependent toxic responses to the CuO nanoparticle insult. In conclusion, toxic responses of the nanoparticles are complex, and they warrant further in vivo studies.

Original languageEnglish (US)
Title of host publication2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
Pages670-673
Number of pages4
StatePublished - 2007
Event2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007 - Santa Clara, CA, United States
Duration: May 20 2007May 24 2007

Publication series

Name2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
Volume2

Other

Other2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007
CountryUnited States
CitySanta Clara, CA
Period5/20/075/24/07

Keywords

  • Cytotoxicity
  • H4 neuroglioma cell
  • High-content cell imaging
  • Metal oxide nanoparticles
  • SH-SY5Y neuroblastoma cell

ASJC Scopus subject areas

  • Mechanical Engineering

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