TY - GEN
T1 - Differential cytotoxicity of metal oxide nanoparticles
AU - Chen, J.
AU - Zhu, J.
AU - Cho, H. H.
AU - Cui, K.
AU - Li, F.
AU - Zhou, X.
AU - Rogers, J. T.
AU - Wong, S. T.C.
AU - Huang, X.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007
Y1 - 2007
N2 - Concerns about potential health hazard of nanomaterials are growing. To determine the potential toxicity of metal oxide nanoparticles, human SH-SY5Y neuroblastoma and H4 neuroglioma cells were exposed to Fe2O 3, CuO, and ZnO nanoparticles and their metal ion counterparts (Fe3+, Cu2+, and Zn2+) at a concentration range of 0.01-100 μM for 48 hours, under the cell culture conditions: 95% O 2, 5% CO2, 85% humidity, 37°C. Their ensemble cell viability was determined by MTS cell proliferation assays. A live/dead cell assay was also performed, and cellular images were acquired by a high-content fluorescence microscope and quantified by a novel computerized image analysis protocol. Our data indicated that exposure of these nanoparticles induced differential toxic effects in both SHSY5Y and H4 cells, and the cells had dose-dependent toxic responses to the CuO nanoparticle insult. In conclusion, toxic responses of the nanoparticles are complex, and they warrant further in vivo studies.
AB - Concerns about potential health hazard of nanomaterials are growing. To determine the potential toxicity of metal oxide nanoparticles, human SH-SY5Y neuroblastoma and H4 neuroglioma cells were exposed to Fe2O 3, CuO, and ZnO nanoparticles and their metal ion counterparts (Fe3+, Cu2+, and Zn2+) at a concentration range of 0.01-100 μM for 48 hours, under the cell culture conditions: 95% O 2, 5% CO2, 85% humidity, 37°C. Their ensemble cell viability was determined by MTS cell proliferation assays. A live/dead cell assay was also performed, and cellular images were acquired by a high-content fluorescence microscope and quantified by a novel computerized image analysis protocol. Our data indicated that exposure of these nanoparticles induced differential toxic effects in both SHSY5Y and H4 cells, and the cells had dose-dependent toxic responses to the CuO nanoparticle insult. In conclusion, toxic responses of the nanoparticles are complex, and they warrant further in vivo studies.
KW - Cytotoxicity
KW - H4 neuroglioma cell
KW - High-content cell imaging
KW - Metal oxide nanoparticles
KW - SH-SY5Y neuroblastoma cell
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M3 - Conference contribution
AN - SCOPUS:34547977370
SN - 1420063421
SN - 9781420063424
SN - 1420061836
SN - 9781420061833
T3 - 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
SP - 670
EP - 673
BT - 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
T2 - 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007
Y2 - 20 May 2007 through 24 May 2007
ER -